019-19741

抗Iba1，兔源（免疫細胞化学用）

钙离子作为信号转导通道中的信使，在所有细胞包括神经细胞的生命活动中起重要作用。钙离子与不同的钙结合蛋白结合后进行级联反应。Iba1是能与巨噬细胞和小胶质细胞发生特异性结合的钙结合蛋白，当这些细胞激活后，Iba1的表达量上调，因此Iba1通常作为鉴定小胶质细胞的标记物使用。近来研究发现，小胶质细胞除了提供营养、保护神经的作用外，还被证明可产生NO、TNF-a、IL-1β等物质，因此将小胶质细胞定义为神经系统的巨噬细胞样吞噬细胞，具有重要的免疫细胞作用。当病原菌、病毒入侵神经系统时会起免疫监御的作用。近来研究发现，该细胞可能与神经退行性疾病的发生和发展有关。本产品是可与小胶质细胞发生特异性反应的兔多抗，配合对星形胶质细胞有特异性的GFAP单抗，可进行双染色。

抗原：合成肽（Iba1 的C末端序列）

外观：TBS溶液（1mg/ml）

提纯：抗原亲和提纯

特异性：对小胶质细胞、巨噬细胞特异反应。不会与神经元、星形胶质细胞发生交叉反应。能与人、小鼠、大鼠Iba1反应。

用法：IBA1抗体，兔源（免疫細胞化学用）适合免疫細胞化学。只需1~2μg/ml即可进行实验。

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Summary

This product is for research use only. Do not administer it to human.

Antibodies against Macrophage/Microglia-specific Protein Iba1

Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.

Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.

Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.

Working Concentration: Immunocytochemistry 1-2 micro-g/mL

Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

Package Size: 50 ug (100 uL) (ug: micro-g; uL: micro-L)

References:

Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).

Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).

Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).

Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).

Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

Wako catalog #016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting (50 micrograms (100 microlitters))Working Concentration: Westernblot 0.5 - 1 micrograms/mL 

[Recommended preparations]

Frozen mouse brain tissue or cultured cells should be perfusion-fixed with 4% paraformaldehyde-PBS. Then the tissue section is prepared. After those preparation, the tissue section and cultured cells are immunostained with anti Iba1 antibody.