人癌胚抗原(CEA/CD66)ELISA Kit

ELISA法定量测定人血清、血浆或其它相关液体中CEA含量。

本试剂盒应用双抗体夹心酶标免疫分析法测定标本中CEA水平。用纯化的抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入CEA抗原、生物素化的抗人CEA抗体、HRP标记的亲和素，经过彻底洗涤后用底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成ZZ的黄色。颜色的深浅和样品中的CEA呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），计算样品浓度。

This immunoassay kit allows for the specific measurement of human CEA concentrations in cell culture supernates, serum, and plasma.

Carcinoembryonic antigen (CEA) is a cell-surface glycoprotein with a molecular weight of 180-200kD, that occurs in high levels in colon epithelial cells during embryonic development. Levels of CEA are significantly lower in colon tissue of adults, but can become elevated when inflammation or tumours arise in any endodermal tissue, including the gastrointestinal tract, respiratory tract, pancreas and breast.

An overexpression of CEA protein has been detected in a variety of adenocarcinomas, including gastric, pancreatic, small intestine, colon, rectal, ovarian, breast, cervical and non-small-cell lung cancers. CEA is also expressed by epithelial cells in several non-malignant disorders, including diverticulitis, pancreatitis, inflammatory bowel disease, cirrhosis, hepatitis, bronchitis and renal failure and also in heavy smokers.

Therefore CEA should not be regarded as a tumour-specific marker for the screening of general population for undetected cancers. However, the determination of CEA levels provides important information about patient prognosis, recurrence of tumours after surgical removal and effectiveness of therapy.

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for CEA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CEA present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for CEA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CEA bound in the initial step. The color development is stopped and the intensity of the color is measured.