（66-669）高分子量 markerProtein mixture(原装)
以下是高分子量蛋白聚丙烯酰胺梯度凝胶电泳图：
关于17-0445-01高分子量蛋白（66-669）的电泳说明：17-0445-01高分子量蛋白（66-669）是在浓度5–12.5%聚丙烯酰胺梯度胶中进行电泳。10ul的量加入2倍体积的稀释液进行实验。 HMW Calibration Kit proteins and various protein samples
run on a 5–12.5% polyacrylamide gradient gel. 10ul of a two-fold
dilution of the HMW calibration proteins (leftmost lane) and various
protein samples were run on a 1mm thick 5–12.5% gradient gel.
Electrophoresis buffer system: The Tris-Glycine, Tris-Chloride
discontinuous system of Ornstein and Davis was used (8) (see also
the Hoefer™ Protein Electrophoresis Applications Guide).
Electrophoresis conditions: the gel was run at 15 mA constant
current on a Hoefer SE260 mini-vertical apparatus until the
bromophenol blue dye front exited the gel.
Staining: The gel was stained with Coomassie™ Blue R350.
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