产品库

orb11064 MMP9 antibody抗体

产品信息

Category

Primary antibodies

Product name

MMP9 antibody

Catalog Number

orb11064

Host

Rabbit

Clonality

Polyclonal

Tested applications

IHC-P, IF/ICC, P-ELISA

Dilution range

P-ELISA: 1:500-1000, IHC-P: 1:400-800, IF/ICC: 1:100-500

Concentration

- 5 μg (in 100 μl): 0.05 mg/ml

- 20 μg (in 100 μl): 0.2 mg/ml

- 100 μg (in 200 μl): 0.5 mg/ml

- 200 μg (in 400 μl): 0.5 mg/ml

Reacts with

Human, Mouse, Rat

UniProt ID

P14780 (Human); P41245 (Mouse)

NCBI

NP_004985.21; NM_004994.21

Entrez

4318

Conjugation

Unconjugated

Immunogen

KLH conjugated synthetic peptide derived between 621-697 amino acids of human MMP9

Purity

Affinity purified by Protein A

MW

78 kDa

Isotype

IgG

Form

Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol

IHC notes

Formalin-fixed paraffin-embedded tissue sections were subjected to antigen retrieval by treatment with 0.01 M Tris-HCl (pH 9.0), followed by incubation with primary MMP9 Antibody (Biorbyt; diluted 1:100)

Application notes

Western Blot: Load 10 ug protein and block with 5% milk, MMP9 antibody is diluted 1:1000 This antibody can be used for WB, the correct bands. Predicted weight: 64/82/92kDa Tested weight: 64/82/92kDa

References

Role of fibulin-5 in metastatic organ colonization.

Storage

Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
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