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active+pro Caspase 3 Rabbit Monoclonal Antibody

产品信息
  • active+pro Caspase 3 Rabbit Monoclonal Antibody
    产品编号 产品名称 产品包装 产品价格
    LM1675 active+pro Caspase 3 Rabbit Monoclonal Antibody 50μl 618.00
     
    来源 用途 交叉反应性 分子量
    Rabbit WB, IP, IHC H 35.0kDa
           WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
    Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
    Immunoprecipitation Assay.
           H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
    Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 
           
    配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
           建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
    WB IP IF IHC ICC FC ELISA ChIP
    1:1,000-1:2,000 1:10-1:50 - 1:50-1:200 - - - -
    抗体详细信息如下:
    About this Antibody
    Name active+pro Caspase 3 Rabbit Monoclonal Antibody
    Category Rabbit Monoclonal Antibody (RabMAb); Primary antibody
    Isotype IgG
    Purification Affinity purification
    About the Immunogen
    Immunogen Recombinant protein
    Gene ID 836(Human)
    SwissProt P42574(Human)
    Synonyms CPP32; CPP32B; SCA-1
    Category Apoptosis
    Background Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
    包装清单:
    产品编号 产品名称 包装
    LM1675 active+pro Caspase 3 Rabbit Monoclonal Antibody 50μl
    LM050 Western一抗稀释液 50ml
    - 说明书 1
    保存条件:
           active+pro Caspase 3 Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
    注意事项:
           如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
           回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
           提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得ZJ的检测效果,请考虑使用上述检测专用的一抗稀释液。
           本产品仅限于专业人员的科学研究用,不得用于临床诊断或ZL,不得用于食品或药品,不得存放于普通住宅内。
           为了您的安全和健康,请穿实验服并戴一次性手套操作。


     
    温馨提示:不可用于临床ZL。
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