pHrodo™ Red Zymosan Bioparticles™ Conjugate for Phagocytosis
货号P35364 Invitrogen™原装描述New pH-sensitive pHrodo® Red dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® Red dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye.
• Specific detection of phagocytosis and endocytosis
• Reduced signal variability and improved timing in sensitive experiments
• Multiplex with green dyes such as GFP, Fluo-4, or calcein
The fluorescence of the novel pHrodo® Red dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes.
Use the ready-made pHrodo® Red Zymosan BioParticles® Conjugate in imaging or flow applications, or pHrodo® Red SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice.
In addition, pHrodo® dye is also available in green color, with
E. coli, zymosan,
S. aureus, and dextran 10,000 conjugates.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.规格
| Color: | Red |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 560⁄585 |
| For Use With (Equipment): | Attune® Acoustic Focusing Cytometer, Confocal Microscope, Floid® Cell Imaging System, Flow Cytometer, Fluorescence Microscope, High Content Instrument |
| Label or Dye: | pHrodo™ Red |
| Product Line: | pHrodo™ |
| Product Size: | 5 x 1 mg |
| Species: | S. cerevisiae |
| Shipping Condition: | Room Temperature |
内容及储存Store at -20 °C, dessicate and protected from light.内吞和吞噬的成像检测
pHrodo™ BioParticles 偶联物
成品pHrodo™ E.coli和S. aureus BioParticles偶联物同样也可直接用于吞噬的流式细胞分析。
| 指示剂 | 大小 | 目录号 |
| 用于吞噬检测的pHrodo™ Red Zymosan A BioParticles偶联物 | 5 x 2 mg | P35364 |
| 用于吞噬检测的pHrodo™ Green Zymosan A BioParticles偶联物 | 5 x 2 mg | P35365 |
| 用于吞噬检测的pHrodo™ Red E.coliBioParticles偶联物 | 5 x 2 mg | P35361 |
| 用于吞噬检测的pHrodo™ GreenE.coliBioParticles偶联物 | 5 x 2 mg | P35366 |
| 用于吞噬检测的pHrodo™ Red S. sureusBioParticles偶联物 | 5 x 2 mg | A10010 |
| 用于吞噬检测的pHrodo™ GreenS. aureusBioParticles偶联物 | 5 x 2 mg | P35367 |
pHrodo™ Dextran偶联物
成品pHrodo™ dextran偶联物也可直接用于基于微孔板的荧光内吞检测
| 指示剂 | 大小 | 目录号 |
| 用于吞噬检测的pHrodo™ Red dextran 10,000 MW | 0.5 mg | P10361 |
| 用于内吞检测的pHrodo™ Green dextran 10,000 MW | 0.5 mg | P35368 |
pHrodo™活性染料
活性pHrodo™染料可使您通过巯基或者胺偶联标记任何微生物、抗体或蛋白质。 pHrodo™染料具有对蛋白进行标记的能力，所以您可以将染料结合到特定受体的配体上来研究受体的内化。
| 指示剂 | 大小 | 目录号 |
| 用于pHrodo™吞噬颗粒标记的流式细胞分析试剂盒 | 1 个 | A10026 |
| pHrodo™ Red，succinimidyl ester（pHrodo™SE） | 1 mg | P36600 |
| pHrodo™ Green, STP ester(pHrodo™, SE) | 500 µg | P35369 |
| pHrodo™ Red，Maleimide | 1 mg | P35371 |
| pHrodo™ Green，Maleimide | 1 mg | P35370 |
| pHrodo™ Red微量标记试剂盒 | 1 mg | P35363 |
| pHrodo™ Red生物素（荧光pH感受器） | 1 mg | P35362 |
温馨提示：不可用于临床ZL。
