Mouse Phosphorylated Neurofilament(pNF-H)ELISA kit

For the quantitative in vitro determination of Insulin concentrations in Mouse culture supernates, serum, plasma and tissue.
1. Serum-Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 xg.Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. 
2. Plasma-Collect plasma using EDTA or Citric acid sodium as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection. Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles. 
3. Cell culture fluid and other biological fluids-Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.样本处理及要求
1. 血清-用采血管收集血液，室温血液自然凝固30分钟，1000 xg离心15分钟左右，收集上清，尽早进行实验，或者分装后-20°C 或 -80°C 保存
2. 血浆-收集好的血浆根据要求选择EDTA或者柠檬酸钠作为抗凝剂，2-8°C 1000 xg离心15分钟左右，30分钟内收集好，-20°C 或 -80°C 保存，避免反复冻融
3. 细胞上清及相关液体-收集好的样本离心后应尽快实验，或者分装后-20°C 或 -80°C 保存，避免反复冻融