磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书

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英文名称  Anti-phospho-Ron/MST1R(Tyr1238+Tyr1239) 
中文名称  磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书
别    名  MST1R(phospho S1238+S1239); c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d’origine nantais (RON); RON; RON protein tyrosine kinase; RON variant E2E3; RON_HUMAN; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase. 
浓    度  1mg/1ml 
规 格  0.1ml/100μg
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat
产品类型  一抗  磷酸化抗体   
研究领域  细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶  
蛋白分子量  predicted molecular weight: 30/119/150kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthesised phosphopeptide derived from human MST1R around the phosphorylation site of Tyr1238+Tyr1239 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 MST1R/Ron, a HGF Receptor/MET-type protein kinase, mediates the biological activities of macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35-kD (alpha) and 150-kD (beta) subunits. Ligand binding results in tyrosine phosphorylation of the beta chain. In knockout studies, MST1R/RON (-/-) mice failed to survive past the periimplantation period. The MST1R/RON gene has been mapped to 3p21, a region of frequent deletion or mutation in small cell lung and renal carcinoma, and has been implicated in the progression of several epithelial cancers. Ron expression has been documented in many normal human tissues. ESTs have been isolated from several tissue libraries, including normal colon, mouth, prostate, and testis and cancerous colon, prostate, stomach, and uterus.
Function : Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.
Subunit : Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion.
Subcellular Location : Membrane; Single-pass type I membrane protein. 
Tissue Specificity : Expressed in colon, skin, lung and bone marrow.
Post-translational modifications : Proteolytic processing yields the two subunits.
Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site. 
Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.
Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain. 
Contains 1 Sema domain. 
Database links : UniProtKB/Swiss-Prot: Q04912.2
  磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书储存温度：
 存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下，抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融，因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏，或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀，就应该存放于室温下的叠氮钠溶液中。
 磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书的分类：
按作用对象，可将其分为抗毒素、KJ抗体、抗病毒抗体和亲细胞抗体（能与细胞结合的免疫球蛋白，如1型变态反应中的lgE反应素抗体，能吸附在靶细胞膜上）；按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类；按抗体的来源，可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白，是改变了的球蛋白分子。由特异性抗原刺激产生，抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用，使淋巴细胞中的B细胞分化增殖而形成浆细胞，浆细胞可产生分泌抗体。
WH 081 7-methoxynaphthyl isomer （1 mg）    （7-methoxy-1-naphthalenyl）（1-pentyl-1H-indol-3-yl）-methanone； JWH 164； JWH 081 7-methoxynaphthyl isomer
JWH 424 （25 mg）    （8-bromonaphthalen-1-yl）（1-pentyl-1H-indol-3-yl）methanone； JWH 424
Linoleoyl Ethanolamide （10 mg）    N-（2-hydroxyethyl）-9Z，12Z-octadecadienamide； Linoleoyl Ethanolamide
9（E），11（E）-Conjugated Linoleic Acid （50 mg）    9E，11E-octadecadienoic acid； 9E，11E-CLA|Isolinoleic Acid|Mangold’s acid； 9（E），11（E）-Conjugated Linoleic Acid
VU0360223 （1 mg）    3-fluoro-5-（2-methyl-5-benzothiazolyl）-benzonitrile； CAY10651； VU0360223
Fatostatin （hydrobromide） （25 mg）    4-[4-（4-methylphenyl）-2-thiazolyl]-2-propyl-pyridine， hydrobromide； 125B11； Fatostatin （hydrobromide）
Dextromethorphan （hydrobromide hydrate） （500 mg）    （9α，13α，14α）-3-methoxy-17-methyl-morphinan， hydrobromide monohydrate； DXM； Dextromethorphan （hydrobromide hydrate）
3，4-MDEA （hydrochloride） （50 mg）    N-ethyl-α-methyl-1，3-benzodioxole-5-ethanamine， monohydrochloride； 3，4-Methylenedioxyethylamphetamine|Eve|MDE； 3，4-MDEA （hydrochloride）
磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书Valsartan （100 mg）    N-（1-oxopentyl）-N-[[2’-（2H-tetrazol-5-yl）[1，1’-biphenyl]-4-yl]methyl]-L-valine； CGP 48933|Diovan|Tareg； ValsartanPhospho-TAK1(Thr184)  磷酸化转化生因子β活化激酶1规格: 0.1mlVTN/Vitronectin  玻璃粘连蛋白抗体规格: 0.1ml
TLR9/CD289  Toll样受体9抗体规格: 0.1mlFAAH1  脂肪酸酰胺水解酶1抗体规格: 0.2ml
TNFRSF14/HVEM  瘤坏死因子受体超家族成员14抗体规格: 0.2mlBTG3  高表达神经上皮蛋白BTG3抗体规格: 0.2ml
SMURF1  Smad蛋白E3泛素连接酶1抗体规格: 0.2mlPSD93  突触后密度蛋白93抗体规格: 0.2ml
TSPAN1  四分子交联体1抗体（四旋蛋白）规格: 0.2mlPCDHA10/CNRN8  钙粘蛋白相关神经受体8抗体规格: 0.2ml
KCTD5  钾离子通道四聚体结构域蛋白5抗体规格: 0.2mlStanniocalcin 1  斯钙素抗体规格: 0.1ml
Orai2  跨膜蛋白Orai2抗体规格: 0.2mlCD44v6  CD44V6抗体规格: 0.1ml
phospho-NFKB p65(Ser536)  磷酸化细胞核因子抗体规格: 0.1mlRPRD1A/P15RS  细胞周期依赖性激酶YZ相关蛋白抗体规格: 0.2ml
phospho-c-Raf(Ser338/Tyr340)  磷酸化原基因c-Raf抗体规格: 0.1mlMASP  甘露聚糖结合凝集素丝氨酸肽酶1抗体规格: 0.2ml
磷酸化原癌基因c-Met相关酪氨酸激酶抗体说明书按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类。
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来，聚成一堆便于巨噬细胞的吞噬；
IgG抗体激活补体，中和多种毒素。IgG持续的时间长，是 WY能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳，使新生儿得 到保护；
IgA抗体进入身体的黏 膜表面，包括呼吸、消化、生殖等管道的黏膜，中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中，是在母乳中含量最多，最为重要的一类抗体；
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后，嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体；
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上，可能与B细胞的分化有关。（IgD于1995年从人瘤蛋白中发现，分子量为175kD，主要由扁桃体、脾等处浆细胞产生，人血清中IgD浓度为3～40μg/ml,不到血清总Ig的1%，在个体发育中合成较晚。IgD铰链区很长，且对蛋白酶水解敏感，因此IgD半衰期很短，仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段，除了表达SmIgD，抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时，SmIgD逐渐消失。