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MicroVue Helical Peptide EIA**

产品信息
  • MicroVue Helical Peptide EIA**

    For Research Use Only. Not for use in diagnostic procedures.

    The MicroVue Helical Peptide is a test to measure type I collagen helical peptide α1(I) 620-633 in urine, a marker for researching collagen degradation.

    Type I collagen is degraded during bone resorption, releasing into circulation peptides of various molecular weights that are further degraded and/or released into urine. A peptide consisting of residues 620-633 derived from the helical region of the α1 chain was isolated from the urine of a patient with Paget's disease.
     

    Features & Benefits
    Feature Benefit
    Product Specifications
    Format

    ELISA
    Test/kit

    96 wells/plate
    Sample type

    Urine
    Performance Characteristics

    Method: Competitive
    Analyte: Helical Peptide α1(I) 620-633
    Specimen: Urine
    Specimen Volume: 20 µL
    LOD*: 8 µg/L
    LLOQ*: 20.2 µg/L
    HLOQ*: 2514 µg/L
    Precision CVs within-run: 4%-8%
    Precision CVs between-run: 7%-17%
    Incubation Time: 19-25 Hours
    Cross-reactivity: Helical peptide α1(I) 620-633 100% Intact collagen I, II, III, IV < 1% Homologous peptides (see Package Insert)

    *LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification

    About the AssayFormat

    96-well microplate with reagents sufficient to test 40 samples in duplicate

    Antibody Specificity

    Helical peptide α1(I) 620-633; no reactivity with intact collagen types I, II, III, IV or mixtures of amino acids; 90% reactivity with α1(III) homologous peptide; no reactivity with α1(I) or α2(II) homologous peptides

    Species Reactivity

    Rat, Mouse, Rabbit, Guinea Pig, Cat, Dog, Pig, Goat, Sheep, Cow, Horse, Rhesus Macaque, Cynomolgus Monkey, Baboon, Chimpanzee, Human Cell Culture

    Assay Steps

    Add 20 µL Standards, Controls, and samples
    Add 150 µL Enzyme Conjugate

    • Incubate 18-24 hours at 2°C to 8°C
    • Wash 3 times with 1x Wash Buffer
    • Add 150 µL Substrate
    • Incubate 1 hour at room temperature
    • Add 100 µL Stop Solution
    • Read absorbance at 405 nm
    • Data Reduction: 4 Parameter
    • Correct for creatinine concentration
    Ordering Information
         
    Catalog Number Description Kit Size / Case Size
    8022

    ELISA Kit

    		 96 Wells/Plate
    Product Documentation
    Package Insert
    SDS
    Technical Data Sheet
    Technical Bulletin - ELISA Wash Technique
    温馨提示：不可用于临床ZL。
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