pIRES-EGFP

质粒名称: pIRES-EGFP
其他名称:
质粒大小（bp）: 5162
抗性: Ampicillin
质粒类型: 哺乳动物细胞表达载体；双顺反子载体；荧光报告载体
是否病毒: 非病毒
启动子:CMV
稳转或瞬转: Stable
组成型或诱导性: Constitutive
表达水平:高
蛋白标签:EGFP
5′ 测序引物及序列: CMV-F: 5′-CGCAAATGGGCGGTAGGCGTG-3′

3′ 测序引物及序列:EGFP-N: 5′-CGTCGCCGTCCAGCTCGACCAG-3′
GenBank号:
其他属性:通过一个转录mRNA，能够同时表达EGFP蛋白和一个目的基因。
质粒图谱:

质粒序列:

LOCUS       pIRES-EGFP              5162 bp    DNA     circular     16-FEB-2014SOURCE        ORGANISM  COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     promoter        232..820                     /vntifkey="29"                     /label=CMV\Promoter     misc_feature    909..974                     /vntifkey="21"                     /label=MCS     intron          974..1269                     /vntifkey="15"                     /label=Synthetic\Intron\(IVS)     RBS             1299..1884                     /vntifkey="32"                     /label=IRES     CDS             1905..2621                     /vntifkey="4"                     /label=EGFP     polyA_signal    2636..2913                     /vntifkey="25"                     /label=poly\A     rep_origin      3343..4016                     /vntifkey="33"                     /label=Col\E1\Origin     CDS             complement(4168..5026)                     /vntifkey="4"                     /label=AmpcillinBASE COUNT     1237 a      1371 c      1325 g      1229 t ORIGIN        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg        61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg       121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc       181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt       241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata       301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc       361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc       421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt       481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt       541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca       601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg       661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc       721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg       781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca       841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc       901 gagctcggat cgatatctgc ggccgcgtcg acggaattca gtggatccac tagtaacggc       961 cgccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc      1021 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat      1081 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa      1141 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact      1201 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac      1261 tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa      1321 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgtgattttc      1381 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac      1441 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt      1501 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg      1561 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata      1621 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga      1681 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt      1741 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc      1801 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac      1861 acgatgataa gcttgccaca acccgggatc caccggtcgc caccatggtg agcaagggcg      1921 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc      1981 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga      2041 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga      2101 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca      2161 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca      2221 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc      2281 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact      2341 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact      2401 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga      2461 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt      2521 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga      2581 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc cctagagctc      2641 gctgatcagc ctcgactgtg cctctagttg ccagccatct gttgtttgcc cctcccccgt      2701 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat      2761 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag      2821 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc      2881 ttctgaggcg gaaagaacca gctggggctc gagtgcattc tagttgtggt ttgtccaaac      2941 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat      3001 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac      3061 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa      3121 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat      3181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc      3241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg      3301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag      3361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc      3421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag      3481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga      3541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc      3601 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg      3661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt      3721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca      3781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca      3841 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag      3901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca      3961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg      4021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa      4081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta      4141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag      4201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga      4261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac      4321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc      4381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta      4441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac      4501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat      4561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa      4621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg      4681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag      4741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc      4801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct      4861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat      4921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg      4981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc      5041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta      5101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg      5161 tc