This assay is a two-site ELISA. The micro-plate is pre-coated with a monoclonal antibody against insulin. Standards and samples are added into the wells and co-incubated with a monoclonal antibody conjugated to horseradish peroxidase (HRP) enzyme. After wash step to remove any unbound substances, TMB substrate is added and colour develops in proportion to the amount of insulin bound initially. The assay is stopped and the optical density of the wells determined using a micro-plate reader. Since the increases in absorbance are directly proportional to the amount of captured insulin, the unknown sample concentration can be interpolated from a reference curve included in each assay. 产品名:Ultra sensitive mouse Insulin ELISA kit 货号:orb54818 产品中文名:Ultra sensitive mouse Insulin ELISA 试剂盒 产品详情链接:http://www.biorbyt.com.cn/detail-page.php?q=orb54818 上海妍琦生物科技有限公司(www.biorbyt.com.cn )为英国品PaiBiorbyt(www.biorbyt.com )代理商。负责biorbyt品Pai产品在ZG市场的推广、销售及售后服务。为您提供各种biorbyt原装进口产品,主推四大类: