Anti-SOCE-Associated Regulatory Factor

Anti-SOCE-Associated Regulatory FactorStore-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, Transmembrane protein 66, TMEM66, SARAF
Cat #: ACC-0067
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IFC, IC, IP, IH
Reactivity: H, M, RApplication key:CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blotSpecies reactivity key:H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SARAF. Anti-SOCE-Associated Regulatory Factor antibody (#ACC-067) can be used in western blot analysis. It has been designed to recognize SARAF from human, rat and mouse samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat pancreas (lanes 1 and 2), MS1 cells (lanes 3 and 5) and PANC1 cells (lanes 4 and 6) lysates:
1, 3, 4. Anti-SOCE-Associated Regulatory Factor antibody (#ACC-067), (1:500).
2, 5, 6. Anti-SOCE-Associated Regulatory Factor antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)ELGLSKLKESGKHQS, corresponding to amino acid residues 141-155 of rat SOCE-associated regulatory factor (Accession Q6AYN2). Intracellular, lumen.

HomologyMouse - 13/15 amino acid residues identical; human - 11/15 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

SOCE-associated regulatory factor (SARAF) is a regulatory protein of cellular Ca²⁺ homeostasis, encoded by the TMEM66 gene. SARAF is an ER resident protein, which responds to cytosolic Ca²⁺ elevation after ER Ca²⁺ refilling.

SARAF is highly conserved in vertebrates, and in mammals it is ubiquitously expressed with high transcript levels in the immune and neuronal tissues. SARAF structure contains validated signal peptide and a putative single membrane spanning domain, a serine-proline rich domain and arginine rich regions followed by a cluster of basic residues at its C-terminal tail₁.

Store-operated Ca²⁺ entry (SOCE) is an important Ca²⁺ influx pathway across plasma membrane in many non-excitable cells. In this pathway, intracellular Ca²⁺ release through IP3 receptor stimulated by a variety of agonists, results in reduction of Ca²⁺ concentration in the lumen of endoplasmic or sarcoplasmic reticulum. SARAF negatively regulates store operated calcium entry into cells and protects cells from calcium overfilling^1,2.

STIM, Ca²⁺ sensor, and Orai, the channel pore forming subunit proteins have been identified as the essential components enabling the reconstitution of Ca²⁺ release-activated Ca²⁺ channels that mediate SOCE. Knock-out of Orai1 or STIM1 in mice and mutations evoke SOCE in lymphocytes and other cells^2,3.

Several findings show that dysregulation of Ca²⁺ homeostasis is associated with a plethora of pathological conditions including neurodegenerative diseases, skeletal, muscular and cardiovascular disorders². Inherited defects in SOCE due to mutations in genes of the Ca²⁺ release-activated Ca²⁺ (CRAC) channel complex causes patients to suffer from a severe form of immunodeficiency that is due to defects in the function of T cells, NK cells and potentially other immune cells³_.

References

1. Palty, R. et al. (2012) Cell 149, 425.
2. Pan, Z. et al. (2014) BMB Rep. 47, 69.
3. Feske, S. (2011) Ann. N Y Acad. Sci. 1238, 74.