Guinea pig antiserum against parvalbumin

Product: Guinea pig antiserum against parvalbumin
Code No: GP72
Form: lyophilized antiserum. No preservatives. 
Quantity: 100 µl.  
Reconstitution: with 100 µl of bi-distilled water.
Description:
This   antibody   was   raised   against   recombinant   mouse   parvalbumin.   GP72   reacts 
specifically  with   parvalbumin   on   immunoblots   of   extracts   of   tissue   originating   from 
human,  monkey,   guinea  pig,   rabbit,   rat,  mouse  and   chicken   (Fig.  1).  The  antibody 
specifically localizes parvalbumin using free-floating or mounted sections of brain (Fig.  
2),  kidney and muscles of  probably all  vertebrates The antiserum does not  stain  the 
brain of parvalbumin-KO mice. 
Spectacular  staining  found  in  immunohistochemistry on  floating sections of  4% PFA-
fixed specimens. 
Fig. 1: Immunoblot of brain extracts of C57/Bl6 and of 
parvalbumin knock-out mice. The GP72 antiserum lack 
reaction with calretinin and calbindin D-28k*
Working dilutions:
Immunohistochemistry:   1:3'000   -   1:10'000,   when   performed   with   the   avidin-biotin 
method.