pLVX-Hom-1

pLVX-Hom-1载体基本信息

出品公司:	Ybscience
载体名称:	pLVX-Hom-1
质粒类型:	慢病毒载体；双顺反子载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	8478 bp
5' 测序引物及序列:	CMV Forward： CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	DmrB (C、N-端均可）
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素（Puromycin）
克隆菌株:	Stbl3
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	慢病毒载体pLVX-Hom-1组成型表达DmrB融合蛋白； 外源基因与嘌呤霉素抗性基因以双顺反子的形式表达。
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒

 载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       pLVX-Hom-1   8478 bp    DNA   circular             5-OCT-2011COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comCOMMENT     Catalog Nos. 635072, 635073      FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=packaging\signal     misc_feature    2028..2151                     /label=cPPT/CTS     misc_feature    4400..4991                     /label=WPRE     rep_origin      complement(6301..6971)                     /label=pUC\ori     CDS             complement(7116..8112)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     LTR             5195..5831                     /label=3'LTR     misc_feature    3179..3754                     /label=IRES     CDS             3787..4386                     /label=Puro(R)     misc_feature    2821..3153                     /label=DmrB     misc_feature    2185..2787                     /label=pCMVBASE COUNT     2169 a      2111 c      2190 g      2008 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa      2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt      2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc      2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc      2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca      2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta      2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa      2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc      2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt      2821 atggcttcta gaggagtgca ggtggagact atctccccag gagacgggcg caccttcccc      2881 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt      2941 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc      3001 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata      3061 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact      3121 ctcgtcttcg atgtggagct tctaaaactg gaaagcggcc gcggatccta cgtatgaccg      3181 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg      3241 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg      3301 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg      3361 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca      3421 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct      3481 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca      3541 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa      3601 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg      3661 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg      3721 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga      3781 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg      3841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac      3901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg      3961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg      4021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg      4081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc      4141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt      4201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc      4261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc      4321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt      4381 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg      4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta      4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct      4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt      4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac      4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg      4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac      4801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg      4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct      4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc      4981 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt      5041 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag      5101 gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt      5161 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg      5221 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca      5281 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct      5341 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt      5401 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt      5461 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa      5521 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg      5581 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg      5641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag      5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc      5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa      5821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat      5881 gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag      5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt      6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc      6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc      6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct      6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca      6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac      6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt      6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg      6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc      6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc      6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc      6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac      6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt      6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct      6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc      6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt      6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc      6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg      7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca      7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca      7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag      7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac      7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc      7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct      7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc      7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg      7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc      7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat      7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag      7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat      7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg      7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca      7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga      7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc      7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata      8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg      8101 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa      8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg      8221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc      8281 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt      8341 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta      8401 tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg      8461 tactacaaac ttagtagt