Beckman A63881 Agencourt AMPure XP 60ml 现货供应

产品特征：

› 200bp以上的核酸回收率高达90%以上，核酸长度最小要求100bp › 有效去除dNTP、引物、引物二聚体、盐离子和其它杂质 › 纯化后的产物在4℃温度下保持7天不被降解 › 双链和单链DNA都能得到纯化 › 长达18个月有效期 › 手工操作和自动化工作站操作皆可

该试剂盒应用于：PCR体系纯化、酶切和连接反应体系纯化

产物适合于下游应用：PCR、Genotyping（SNP detection）、sequencing（Sanger and Next Generation Sequencing）、Cloning、Primer Walking

纯化产物得率高
Agencourt AMPure XP可以结合大于100bp的核酸片段，对于片段长度为10KB的长片段，也能获得比传统过柱法高得多的得率。

图1：经过3种纯化试剂纯化后的产物得率对比：分别是200bp、800bp和10KB，纯化所用的试剂分别为Agencourt AMPure XP、QIAquick 和QuickStep 过柱法纯化试剂盒。

操作过程

• 1. 加入AMPure XP 磁珠试剂
  2. 磁珠与核酸结合
  3. 结合有核酸的磁珠与杂质分离
  4. 乙醇洗涤
  5. 加入洗脱液
  6. 转移洗脱产物

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features

• High recovery of amplicons, greater than 100 bp
• Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
• Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
• Efficient recovery of double stranded and single stranded DNA templates
• Consistent recovery throughout the kit’s 12 month shelf life
• Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Process Overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NX^P and FX^P for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

Ordering Information

To request a quote or make an inquiry use the on-line contact form. In the United States and Canada call +1 800 361 7780. Outside the United States and Canada consult the International Distributors list.

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