Alltima HPC18分析柱的详细资料：C18分析柱Alltima HP 系列是我们在高纯硅胶上使用了的键和相技术得到的产品。这个系列有各种不同的键和相供选择，稳定耐用，性能，特别适用于高难样品的分析。当您使用的是较容易流失的键和相类型如C18 Amide（C18 酰胺基）或Cyano（氰基）时，或者使用的冲洗条件较为苛刻（酸碱性较强，或柱温较高），或者配用的检测器对于键和相流失较为敏感如MS 或ELSD 时，也应该优先选择Alltima HP 以获得更好更稳定的使用效果。
| Alltima HP 键和相规格 |
| 键和相 | 填料基质 | 填料形状 | 填料粒径 | 孔径 | 表面积 | 碳载量 | 键和类型 | 封尾？ | USP代码 |
| C18 | 硅胶 | 球形 | 3, 5μm | 190? | 200m2/g | 12% | 单点型 | 是 | L1 |
| C18 EPS | 硅胶 | 球形 | 3, 5μm | 190? | 200m2/g | 4% | 单点型 | 是 | L1 |
| C18 HiLoad | 硅胶 | 球形 | 3, 5μm | 100? | 450m2/g | 24% | 单点型 | 是 | L1 |
| C18 AQ | 硅胶 | 球形 | 3, 5μm | 100? | 450m2/g | 20% | 单点型 | 是 | L1 |
| C18 Amide | 硅胶 | 球形 | 3, 5μm | 190? | 200m2/g | 12% | 单点型 | 是 | L1 |
| C8 | 硅胶 | 球形 | 3, 5μm | 190? | 200m2/g | 8% | 单点型 | 是 | L7 |
| Cyano (CN) | 硅胶 | 球形 | 3, 5μm | 190? | 200m2/g | 4% | 单点型 | 是 | L10 |
| Silica | 硅胶 | 球形 | 3, 5μm | 100? | 450m2/g | ―― | ―― | 无 | L3 |
| HILIC | 硅胶 | 球形 | 1.5, 3, 5μm | 120? | 230m2/g | ―― | ―― | ―― | L3 |
C18分析住在中性pH7 的条件下检测碱性很强的药物，依然能得到对称性很好的峰形。
Alltima HP C18 柱显示了pH1 和pH10 条件下极强的稳定性。
pH1 酸性柱寿命测试条件：以乙腈：稀硫酸pH 1.0 (50:50)的混合溶液冲洗柱24,000 倍体积，柱温保持在60 摄氏度。上方蓝色线条谱图为经
受酸性条件冲洗后的测试谱图。pH10 碱性柱寿命测试条件：以乙腈：氨水 pH10 (50:50)的混合溶液冲洗柱24,000 倍体积，柱温保持在20 摄氏
度。次上方红色线条谱图为经受碱性条件冲洗后的测试谱图。两谱图与测试进行前的初始谱图（下方的黑色线条谱图）几乎相当。测试Alltima HP 柱和其他柱在MS 检测器上显示的键和相流失情况。左边是未接入柱时的空白值0.82，Alltima HP Silica，C18 和C18 Hiload 柱得到的背景噪音与空白值相当，表明没有可见的键和相流失。市场上其他产品所谓MS 专用柱如“C18 MS 1"和“C18 MS 2"都显示出不同程度的键和相流失（背景噪音高于空白值）。C18 Amide 是 “内嵌"了极性官能团的C18 键和相，容易发生水解，比常规C18 键和相的流失率高，而测试结果表明Alltima? HP C18 Amide 的流失也只相当于“C18 MS 2"，远远低于市场上其他C18 Amide 柱的键和相流失程度。
氰基柱是众所周知的易流失、寿命低的色谱柱。柱寿命测试表明Alltima HP 氰基柱比市场上其他各品Pai的氰基柱的耐用性好得多。分别以酸性条件和碱性条件考验柱，比较测试前后对样品的保留值变化（保留值降低的程度表示键和相流失的程度）。pH1.0 耐酸稳定性测试条件：用硫酸pH1.0：乙腈 50:50 以1mL/min 持续冲洗柱三天。pH7.5 耐碱稳定性测试条件：用20mM K2HPO4 pH7.5：乙腈 50:50 以1mL/min 持续冲洗柱三天。Alltima?HP 氰基柱的保留值降低仅为2％左右，而其它各品Pai氰基柱的保留值降低幅度高达5-30％。C18分析柱Alltima HP 方法开发套装：
每套含三根不同键和相类型但尺寸相同的色谱柱，请按您的液相系统配置、被测样品类型及复杂程度选择合适的套装。按套购买价格更为优惠。
| 套装名称 | 柱规格(i.d. x L) | 柱键和相（及各柱相应货号） | 货号 |
| Kit 1 – C18 mix, 3μm |
| Expedite MS Kit 1 | 2.1 x 20mm | C18, C18 Hi-Load, C18 Amide (87674, 87692, 87728) | 87854 |
| Analytical Kit 1 | 4.6 x 150mm | C18, C18 Hi-Load, C18 Amide (87668, 87686, 87722) | 87850 |
| Rocket Kit 1 | 7 x 53mm | C18, C18 Hi-Load, C18 Amide (87672, 87690, 87726) | 87852 |
| Kit 2 – Alternate mix, 3μm | |
| Expedite MS Kit 2 | 2.1 x 20mm | C18 Hi-Load, C8, CN (87692, 87746, 87764) | 87864 |
| Analytical Kit 2 | 4.6 x 150mm | C18 Hi-Load, C8, CN (87686, 87740, 87760) | 87860 |
| Rocket Kit 2 | 7 x 53mm | C18 Hi-Load, C8, CN (87690, 87744, 87762) | 87862 |
| Kit 3 – Polar mix, 3μm |
| Expedite MS Kit 3 | 2.1 x 20mm | C18 EPS, C18 Amide, CN (87710, 87728, 87764) | 87874 |
| Analytical Kit 3 | 4.6 x 150mm | C18 EPS, C18 Amide, CN (87704, 87722, 87758) | 87870 |
| Rocket Kit 3 | 7 x 53mm | C18 EPS, C18 Amide, CN (87708, 87726, 87762) | 87872 |
C18分析柱 常用Alltima HP 色谱柱和保护柱货号快速获取表：
【有更多规格未列出。如需不同粒径、柱内径或柱长度，请垂询上海宸乔生物科技有限公司】
| 填料 | 柱硬件规格 | 货号 |
| C18, 3μm | Capillary: 0.150 x 150mm | 22155 |
| | Capillary: 0.300 x 150mm | 22158 |
| | Expedite MS: 2.1 x 10mm | 87673 |
| | Expedite MS: 2.1 x 20mm | 87674 |
| | Microbore: 2.1x 50mm | 87504 |
| | Microbore: 2.1 x 150mm | 87670 |
| | Analytical: 4.6 x 50mm | 87826 |
| | Analytical: 4.6 x 150mm | 87668 |
| | Rocket: 7 x 33mm | 87671 |
| | Rocket: 7 x 53mm | 87672 |
| C18, 5μm | Microbore: 2.1 x 150mm | 87681 |
| | Microbore: 2.1 x 250mm | 87682 |
| | Analytical: 4.6 x 150mm | 87679 |
| | Analytical: 4.6 x 250mm | 87680 |
| C18 AQ, 3μm | Capillary: 0.150 x 150mm | 22562 |
| | Capillary: 0.300 x 150mm | 22565 |
| | Expedite MS: 2.1 x 10mm | 87813 |
| | Expedite MS: 2.1 x 20mm | 87814 |
| | Microbore: 2.1 x 150mm | 87810 |
| | Analytical: 4.6 x 50mm | 87832 |
| | Analytical: 4.6 x 150mm | 87808 |
| | Rocket: 7 x 33mm | 87811 |
| | Rocket: 7 x 53mm | 87812 |
| C18 AQ, 5μm | Microbore: 2.1 x 150mm | 87821 |
| | Microbore: 2.1 x 250mm | 87822 |
| | Analytical: 4.6 x 150mm | 87819 |
| | Analytical: 4.6 x 250mm | 87820 |
| C18 EPS, 3μm | Expedite MS: 2.1 x 10mm | 87709 |
| | Expedite MS: 2.1 x 20mm | 87710 |
| | Microbore: 2.1x 50mm | 87508 |
| | Microbore: 2.1 x 150mm | 87706 |
| | Analytical: 4.6 x 50mm | 87833 |
| | Analytical: 4.6 x 150mm | 87704 |
| | Rocket: 7 x 33mm | 87707 |
| | Rocket: 7 x 53mm | 87708 |
| C18 EPS, 5μm | Microbore: 2.1 x 150mm | 87717 |
| | Microbore: 2.1 x 250mm | 87718 |
| | Analytical: 4.6 x 150mm | 87715 |
| | Analytical: 4.6 x 250mm | 87716 |
| C18 Hi-Load, 3μm | Capillary: 0.150 x 50mm | 22190 |
| | Capillary: 0.300 x 50mm | 22193 |
| | Expedite MS: 2.1 x 10mm | 87691 |
| | Expedite MS: 2.1 x 20mm | 87692 |
| | Microbore: 2.1x 50mm | 87506 |
| | Microbore: 2.1 x 150mm | 87688 |
| | Analytical: 4.6 x 50mm | 87827 |
| | Analytical: 4.6 x 150mm | 87686 |
| | Rocket: 7 x 33mm | 87689 |
| | Rocket: 7 x 53mm | 87690 |
| C18 Hi-Load, 5μm | Microbore: 2.1 x 150mm | 87699 |
| | Microbore: 2.1 x 250mm | 87700 |
| | Analytical: 4.6 x 150mm | 87697 |
| | Analytical: 4.6 x 250mm | 87698 |
| C18 Amide, 3μm | Capillary: 0.150 x 50mm | 22255 |
| | Capillary: 0.300 x 50mm | 22258 |
| | Expedite MS: 2.1 x 10mm | 87727 |
| | Expedite MS: 2.1 x 20mm | 87728 |
| | Microbore: 2.1x 50mm | 87510 |
| | Microbore: 2.1 x 150mm | 87724 |
| | Analytical: 4.6 x 50mm | 87829 |
| | Analytical: 4.6 x 150mm | 87722 |
| | Rocket: 7 x 33mm | 87725 |
| | Rocket: 7 x 53mm | 87726 |
| C18 Amide, 5μm | Microbore: 2.1 x 150mm | 87735 |
| | Microbore: 2.1 x 250mm | 87736 |
| | Analytical: 4.6 x 150mm | 87733 |
| | Analytical: 4.6 x 250mm | 87734 |
| C8, 3μm | Capillary: 0.150 x 150mm | 22481 |
| | Capillary: 0.300 x 150mm | 22484 |
| | Expedite MS: 2.1 x 10mm | 87745 |
| | Expedite MS: 2.1 x 20mm | 87746 |
| | Microbore: 2.1x 50mm | 87512 |
| | Microbore: 2.1 x 150mm | 87742 |
| | Analytical: 4.6 x 50mm | 87830 |
| | Analytical: 4.6 x 150mm | 87740 |
| | Rocket: 7 x 33mm | 87743 |
| | Rocket: 7 x 53mm | 87744 |
| C8, 5μm | Microbore: 2.1 x 150mm | 87753 |
| | Microbore: 2.1 x 250mm | 87754 |
| | Analytical: 4.6 x 150mm | 87751 |
| | Analytical: 4.6 x 250mm | 87752 |
| HILIC 1.5μm | Expedite MS: 2.1 x 20mm | 86471 |
| | Rocket: 7 x 33mm | 86467 |
| HILIC 3μm | Capillary: 0.150 x 150mm | 22517 |
| | Capillary: 0.300 x 150mm | 22520 |
| | Expedite MS: 2.1 x 10mm | 86473 |
| | Expedite MS: 2.1 x 20mm | 86475 |
| | Microbore: 2.1 x 50mm | 86461 |
| | Microbore: 2.1 x 150mm | 86463 |
| | Analytical: 4.6 x 50mm | 86460 |
| | Analytical: 4.6 x 150mm | 86462 |
| | Rocket: 7 x 33mm | 86469 |
| | Rocket: 7 x 53mm | 86470 |
| HILIC 5μm | Microbore: 2.1 x 150mm | 86465 |
| | Analytical: 4.6 x 150mm | 86464 |
| | Analytical: 4.6 x 250mm | 86466 |
| Cyano 3μm | Expedite MS: 2.1 x 10mm | 87763 |
| | Expedite MS: 2.1 x 20mm | 87764 |
| | Microbore: 2.1 x 50mm | 87514 |
| | Microbore: 2.1 x 150mm | 87760 |
| | Analytical: 4.6 x 50mm | 87825 |
| | Analytical: 4.6 x 150mm | 87758 |
| | Rocket: 7 x 33mm | 87761 |
| | Rocket: 7 x 53mm | 87762 |
| Cyano 5μm | Microbore: 2.1 x 150mm | 87781 |
| | Microbore: 2.1 x 250mm | 87782 |
| | Analytical: 4.6 x 150mm | 87769 |
| | Analytical: 4.6 x 250mm | 87780 |
| Silica 3μm | Expedite MS: 2.1 x 10mm | 87791 |
| | Expedite MS: 2.1 x 20mm | 87792 |
| | Microbore: 2.1 x 50mm | 87516 |
| | Microbore: 2.1 x 150mm | 87788 |
| | Analytical: 4.6 x 50mm | 87831 |
| | Analytical: 4.6 x 150mm | 87786 |
| | Rocket: 7 x 33mm | 87789 |
| | Rocket: 7 x 53mm | 87790 |
| Silica 5μm | Microbore: 2.1 x 150mm | 87799 |
| | Microbore: 2.1 x 250mm | 87802 |
| | Analytical: 4.6 x 150mm | 87797 |
| | Analytical: 4.6 x 250mm | 87798 |
| 用于保护毛细管柱的卡套与柱芯： |
| 0.100mmID 毛细管柱保护卡套，1 套 | GR-3710E |
| 0.300mmID 毛细管柱保护卡套，1 套 | GR-3710A |
| C18 毛细管保护柱芯 3μm | 0.150 x 10mm | 22578 |
| 0.300 x 10mm | 22579 |
| C18 AQ 毛细管保护柱芯 3μm | 0.150 x 10mm | 22638 |
| 0.300 x 10mm | 22639 |
| C18 Hi-Load 毛细管保护柱芯 3μm | 0.150 x 10mm | 22588 |
| 0.300 x 10mm | 22589 |
| C18 Amide 毛细管保护柱芯 3μm | 0.150 x 10mm | 22598 |
| 0.300 x 10mm | 22599 |
| C8 毛细管保护柱芯 3μm | 0.150 x 10mm | 22648 |
| 0.300 x 10mm | 22649 |
| HILIC 毛细管保护柱芯 3μm | 0.150 x 10mm | 22658 |
| 0.300 x 10mm | 22659 |
| 用于保护分析柱的卡套与柱芯： |
| All-Guard保护柱卡套（含柱间连接件）1 套 | 80101 |
| C18 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87684 |
| 2.1 x 7.5mm, 3/pk | 87683 |
| C18 AQ 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87824 |
| 2.1 x 7.5mm, 3/pk | 87823 |
| C18 EPS 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87720 |
| 2.1 x 7.5mm, 3/pk | 87719 |
| C18 Hi-Load 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87702 |
| 2.1 x 7.5mm, 3/pk | 87701 |
| C18 Amide 保护柱芯 5um | 4.6 x 7.5mm, 3/pk | 87738 |
| 2.1 x 7.5mm, 3/pk | 87737 |
| C8 保护柱芯5μm | 4.6 x 7.5mm, 3/pk | 87756 |
| 2.1 x 7.5mm, 3/pk | 87755 |
| HILIC 保护柱芯5μm | 4.6 x 7.5mm, 3/pk | 86480 |
| 2.1 x 7.5mm, 3/pk | 86479 |
| Cyano 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87784 |
| 2.1 x 7.5mm, 3/pk | 87783 |
| Silica 保护柱芯 5μm | 4.6 x 7.5mm, 3/pk | 87804 |
| 2.1 x 7.5mm, 3/pk | 87803 |
