Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价
- 品牌:百奥莱博
- 型号:北京百奥莱博科技有限公司
- 产地:北京
- 供应商:北京百奥莱博科技有限公司
- 供应商报价:¥130 - 3350
- 标签:Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价、Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价价格、Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价厂家、Rb-luc Reporter gene plasmid、、北京百奥莱博科技有限公司
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北京百奥莱博专业生产销售Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价,我公司供应的分子生物学试剂品类齐全,且具有优势价格,欢迎新老客户垂询订购。
名称:Rb荧光素酶报告基因质粒(Rb抑癌基因质粒)优惠价
编号:YT454
品Pai:百奥莱博
英文名:Rb-luc Reporter gene plasmid
规格:1μg
Rb报告基因质粒是用于检测Rb(retinoblastoma protein)转录YZ活性水平的报告基因质粒。Rb质粒是以pGL6-TA质粒为模板,在其多克隆位点插入了多个Rb结合位点,可以高灵敏度地检测Rb的转录YZ活性水平。Rb可以和E2F等转录因子结合,YZ这些转录因子的激活,YZ细胞周期的进展。Rb同时也可以作为一个YZ性转录因子直接结合到其响应的顺式作用元件,YZ该基因的转录。
pGL6-TA质粒是用于在哺乳动物细胞中进行萤火虫萤光素酶报告基因检测的新一代质粒。该报告基因质粒比Promega公司的pGL3系列有了全面的改进,一方面对于luciferase的编码进行了改进,确保能更好地在哺乳动物细胞中进行表达,同时对整个质粒中所有可以被预测出的可能的转录因子结合位点全部进行了适当的突变处理,在保持原有功能不变的情况下,使各种转录因子在质粒上的非特异性结合降到ZD。
Rb质粒的主要信息如下:
| Base pairs | 5677 |
| Rb response element | 32-91 |
| Minimal TA promoter (pTA) | 114-136 |
| luc2 reporter gene | 178-1830 |
| SV40 late poly(A) signal | 1865-2086 |
| SV40 early enhancer/promoter | 2134-2552 |
| Synthetic neomycin phosphotransferase(Neor) coding region | 2577-3371 |
| Synthetic poly(A) signal | 3396-3444 |
| Reporter Vector primer 4 (RVprimer4) binding region | 3511-3530 |
| ColE1-derived plasmid replication origin | 3768 |
| Synthetic Beta-lactamase (Ampr) coding region | 4559-5419 |
| Synthetic poly(A) signal/transcriptional pause site | 5524-5677 |
| Reporter Vector primer 3 (RVprimer3) binding region | 5626-5645 |

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| About this Antibody | |
| Name | Integrin β1/CD29 Rabbit Polyclonal Antibody |
| Category | Polyclonal antibody(pAb); Primary antibody |
| Isotype | IgG |
| Purification | Peptide affinity purified |
| About the Immunogen | |
| Immunogen | This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to CD29. |
| Gene ID | 3688 |
| SwissProt | P05556 |
| Synonyms | CD29; FNRB; Fibronectin receptor beta subunit; ITB1; Integrin VLA-4 beta subunit; Integrin beta-1 precursor; integrin beta-1 |
| Category | Adhesion/ECM |
| Background | Integrins are transmembrane receptors that mediate the attachment between a cell and its surroundings, such as other cells or the extracellular matrix (ECM). Integrins are obligate heterodimers containing two distinct chains, called the α (alpha) and β (beta) subunits. The molecular mass of the integrin subunits can vary from 90 kDa to 160 kDa. Beta subunits have four cysteine-rich repeated sequences. Both α and β subunits bind several divalent cations. Integrins have two main functions: Attachment of the cell to the ECM and signal transduction from the ECM to the cell. However, they are also involved in a wide range of other biological activities, including immune patrolling, cell migration, and binding to cells by certain viruses, such as adenovirus, echovirus, hantavirus, and foot and mouth disease viruses. Research studies have implicated β1 integrin in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis. |
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