| Catalogue Number | TRP-247 |
| Introduction | Treponema pallidum is a gram-negative spirochaete bacterium and is considered to be metabolically crippled. There are at least four known subspecies: T. pallidum pallidum, T. pallidum pertenue, T. pallidum carateum and T. pallidum endemicum. The helical structure of T. pallidum pallidum allows it to move in a corkscrew motion through viscous mediums such as mucus. Treponema pallidum sub sp. pallidum has one of the smallest bacterial genomes at 1.14 million base pairs (Mb) and has limited metabolic capabilities, reflecting its adaptation through genome reduction to the rich environment of mammalian tissue. |
| Description | The E.Coli derived recombinant 6xHis tag fusion protein is a multimer having a molecular mass of 48kDa and contains the Trp. Pallidum p15 immunodominant regions and six histidines fused at the C- terminus. |
| Purification Method | Treponema Pallidum protein was purified by proprietary chromatographic technique. |
| Purity | Treponema Pallidum protein is >95% pure as determined by SDS- PAGE. |
| Formulation | 70mM Tris-HCl pH 8.0, 50mM NaCl, 50% Glycerol, 1.5 M Urea. |
| Storage | Treponema Pallidum protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Specificity | Immunoreactive with sera of Trp. Pallidum infected individuals. |
| Applications | Treponema Pallidum protein is suitable for ELISA and Western blots, excellent antigen for detection of Trp. Pallidum with minimal specificity problems. |
| Usage | ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
