荧光cy3染料cy3 dye价格 Cy3 maleimide高纯度荧光染料

荧光cy3染料cy3 dye价格 Cy3 maleimide高纯度荧光染料
Cyanine菁染料是近红外荧光染料中最常用的一类荧光染料，细胞和组织的自发荧光在近红外波段最小，能提供更高的特异性和灵敏度，菁染料是二个非区域电荷的氮原子通过聚合搭桥的方式形成的一种分子，以下图片WM诠释：

荧光cy3染料cy3 dye价格 Cy3 maleimide高纯度荧光染料
Cyanine3/5/7-药物分子或小分子多肽用于小鼠活体成像实验
实验步骤如下：
SPF级 BALB/C裸鼠，6～8 周龄, 18～20克, 实验前24 h 自由进食、饮水。
将裸鼠俯卧位平放于小动物多光谱活体成像系统的记录暗箱中。
实验时将Cy7 或Cy7 标记的生物分子或药物DMSO 稀释后,于裸鼠尾静脉注射200μL( 0.5 mg/g) [ZJ用量和时间需要客户根据自己的仪器和药物试剂等条件优化] ,每5min 记录1张动物在体内发射荧光的成像图片,分析荧光药物的分布情况。对照鼠不注射药物，进行同时记录。记录结束后迅速解剖裸鼠的心、肝、脾、肺、肾等脏器，进行成像。
Cy7 检测时激发波长700～770 nm 带通 ,发射波长790 nm 长通。液晶可调谐滤光片扫描范围 780～950 nm ,扫描步进10 nm。曝光时间为500 ms。
不同的药物代谢时间不一样，注射入裸鼠体内，荧光立即分布全身，然后逐步向膀胱聚集，呈现显著的肾排泄的特点一般4~6 小时，快得只有30 分钟;如果是骨骼等部位靶点的Cy7 标记药物，有客户反映一周后活体成像系统仍能检测到荧光成像。

首先我们用不同的活性基团的Cyanine菁染料和相应的活性基团的生物分子或小分子药物发生反应，链接到一起。
 
Cyanine3 NHS ester和带NH2的药物分子连接
Cyanine7 maleimide和带SH的药物分子或生物分子连接
Cyanine5 amine和带NHS的药物分子或小分子连接
Cyanine5.5 azide和Cyanine3.5 alkyne用于连接DNA和oligonucleotides
 
1. Dissolve the protein or other molecule containing thiol to be labeled in degassed buffer (PBS, Tris, HEPES are good, although others buffers containing no thiols can be used) at pH 7-7.5 in plastic vial. Buffer can be degassed by applying vacuum on it for several minutes, or by bubbling through inert gas (nitrogen, argon, or helium). For proteins, good concentration is between 1-10 mg/mL.
 
2. Add an excess of TCEP (tris-carboxyethylphosphine) reagent to reduce disulfide bonds, flush with inert gas, and close. 100x molar excess of TCEP is fine. Keep the mixture for 20 minutes at room temperature.
 
3. Dissolve maleimide in DMSO or fresh DMF (1-10 mg in 100 uL).
 
4. Add dye solution to thiol solution (20x fold excess of dye), flush vial with inert gas, and close tightly. 5. Mix thoroughly, and keep overnight at room temperature, or 4 Celsius. 6. Purify by gel filteration, HPLC, FPLC, or electrophoresis
 
For maleimides with poor aqueous solubility, like most dye maleimides, we recommend use of cosolvent (DMF or DMSO). Maleimides with good aqueous solubility (like sulfo-Cy maleimides) can be dissolved in water. If precipitation occurs, increase content of organic co-solvent in the mixture to achieve better labeling.
荧光cy3染料cy3 dye价格 Cy3 maleimide高纯度荧光染料
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Cyanine3 maleimide
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Cyanine3 NHS ester   1032678-38-8
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Cyanine5 alkyne
Cyanine5 amine
Cy5 amine
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Cyanine5 azide
Cy5 N3
Cyanine5 Boc-hydrazide
Cyanine5 carboxylic acid
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Cyanine5 maleimide
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Cyanine7 amine   1650635-41-8
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Cy7 NH2
Cyanine7 azide
Cy7 azide
Cyanine7 carboxylic acid
Cyanine7 maleimide
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Cyanine7 NHS ester
Sulfo-Cyanine 5 NHS ester
Sulfo-Cy 5 NHS ester
Sulfo-Cyanine3 azide
Sulfo-Cy3 azide
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 maleimide
Sulfo-Cy3 maleimide
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Sulfo-Cy3 NHS ester
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5 azide
Sulfo-Cy5 azide
Sulfo-Cyanine5 carboxylic acid   1121756-16-8
Sulfo-Cyanine5 maleimide
Sulfo-Cy5 maleimide
Sulfo-Cyanine7 azide
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7 NHS ester
Sulfo-Cy7 NHS ester
Cyanine3 alkyne
Cy3 alkyne
Cyanine3 azide
Cy3 azide
Cy3 N3
Cyanine3 carboxylic acid  1144107-76-5
Cy3 carboxylic acid
Cyanine3 hydrazide
Cyanine3 maleimide
Cy3 maleimide
Cyanine3 NHS ester   1032678-38-8
Cy3 NHS ester
Cyanine5 alkyne
Cyanine5 amine
Cy5 amine
Cy5 NH2
Cyanine5 azide
Cy5 N3
Cyanine5 carboxylic acid
Cyanine5 hydrazide 
Cyanine5 maleimide
Cy5 maleimide
Cyanine5 NHS ester  1032678-42-4
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Cyanine7 amine   1650635-41-8
Cy7 amine
Cy7 NH2
Cyanine7 azide
Cy7 azide
Cyanine7 carboxylic acid
Cyanine7 maleimide
Cy7 maleimide
Cyanine7 NHS ester
Cy7 NHS ester
Sulfo-Cyanine 5 NHS ester
Sulfo-Cy 5 NHS ester
Sulfo-Cyanine3 azide
Sulfo-Cy3 azide
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine3 maleimide
Sulfo-Cy3 maleimide
Sulfo-Cyanine3 NHS ester
Sulfo-Cy3 NHS ester
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5 azide
Sulfo-Cy5 azide
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Sulfo-Cyanine5 maleimide
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Sulfo-Cyanine7 azide
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7 NHS ester
Sulfo-Cy7 NHS ester
 荧光cy3染料cy3 dye价格 Cy3 maleimide高纯度荧光染料
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