pMIR-REPORT Luciferase载体
- 品牌：XYbscience
- 型号：信裕生物
- 产地：中国/美国
- 供应商：上海信裕生物科技有限公司
- 供应商报价：¥1000
- 标签：pMIR-REPORT Luciferase载体、pMIR-REPORT Luciferase载体价格、pMIR-REPORT Luciferase载体厂家、pMIR-REPORT Luciferase、、上海信裕生物科技有限公司
产品库
| 质粒类型: | RNAi载体 |
|---|---|
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6470 bp (查看载体序列) |
| 5' 测序引物: | Luc-F, M13-F20 |
| 载体抗性: | Ampicillin (氨苄青霉素) |
| 筛选标记: | Puromycin (嘌呤霉素) |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1399 | pMIR-REPORT Luciferase | 5ug质粒 | ¥1000.00 |
The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.
The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.
载体应用pMIR-REPORT™ Luciferase is designed for cloning and testing putative miRNA binding sites. pMIR-REPORT™ Luciferase can be transfected into mammalian cells to evaluate endogenous miRNA expression, or used to evaluate the up- or down-regulation resulting from the transfection of Pre-miR™ miRNA Molecules or Anti-miR™ miRNA Inhibitor Molecules, respectively.
