pGreenII0049载体

LOCUS       Exported File           7065 bp ds-DNA    circular SYN 14-七月-2016DEFINITION  Agrobacterium binary vector with kanamycin-resistance and luciferase            genes.ACCESSION   .VERSION     .KEYWORDS    pGreenII 0049SOURCE      http://www.biofeng.com/  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 7065)  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.  TITLE     pGreen: a versatile and flexible binary Ti vector for             Agrobacterium-mediated plant transformation.  JOURNAL   Plant Mol. Biol. 2000;42:819-32.  PUBMED    10890530REFERENCE   2  (bases 1 to 7065)  AUTHORS   pGreen website  TITLE     Direct Submission  JOURNAL   Exported 2016-07-14 from SnapGene Viewer 1.5.3            http://www.snapgene.comCOMMENT     This plasmid must be transformed into an Agrobacterium strain that             also carries a vector such as pSoup.FEATURES             Location/Qualifiers     source          1..7065                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     rep_origin      63..498                     /gene="                     "                     /note="pSa ori"                     /note="origin of replication from bacterial plasmid pSa"                     /note="color: #ffff00"     misc_feature    633..655                     /note="LB"                     /note="left border repeat from nopaline C58 T-DNA                      (truncated)"                     /note="color: #ffe4c4"     terminator      679..930                     /note="NOS terminator"                     /note="nopaline synthase terminator and poly(A) signal"                     /note="color: #ffffff"     CDS             complement(970..1764)                     /codon_start=1                     /gene="aph(3')-II (or nptII)"                     /product="aminoglycoside phosphotransferase from Tn5"                     /note="NeoR/KanR"                     /note="confers resistance to neomycin, kanamycin, and G418                      (Geneticin(R))"                     /note="color: #ccffcc"                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"     promoter        complement(1798..1977)                     /note="NOS promoter"                     /note="nopaline synthase promoter"                     /note="color: #000000; direction: LEFT"     CDS             complement(2048..2437)                     /codon_start=1                     /gene="lacZ (truncated)"                     /product="LacZ-alpha fragment of?beta-galactosidase"                     /note="lacZ"                     /note="color: #993366"                     /translation="MTMITPSSELTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI                     PSTSRGGPVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART                     DRPSQQLRSLNGEWREIVNVNVIDT"     primer_bind     2224..2240                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: RIGHT"     promoter        2247..2265                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"                     /note="color: #ffffff; direction: RIGHT"     misc_feature    2274..2381                     /note="MCS"                     /note="pBluescript multiple cloning site"                     /note="color: #99ccff"     promoter        complement(2394..2412)                     /note="T3 promoter"                     /note="promoter for bacteriophage T3 RNA polymerase"                     /note="color: #ffffff; direction: LEFT"     primer_bind     complement(2433..2449)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: LEFT"     protein_bind    2457..2473                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."                     /note="color: #31849b"     promoter        complement(2481..2512)                     /note="lac promoter"                     /note="promoter for the E. coli lac operon"                     /note="This reverse directional feature has 3 segments:                     ???1:?2481?..?2487?/?#ffffff?/?-10                     ???2:?2488?..?2506?/?#ffffff                     ???3:?2507?..?2512?/?#ffffff?/?-35"     polyA_signal    2787..2963                     /note="CaMV poly(A)"                     /note="cauliflower mosaic virus polyadenylation signal"                     /note="color: #a6acb3"     CDS             complement(3079..4731)                     /codon_start=1                     /gene="luc"                     /product="firefly luciferase"                     /EC_number="                     "                     /note="luciferase"                     /note="color: #ffcc00"                     /experiment="                     "                     /protein_id="                     "                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"     promoter        complement(4742..5087)                     /note="CaMV 35S promoter"                     /note="strong constitutive promoter from cauliflower mosaic                     virus"                     /note="color: #000000; direction: LEFT"     misc_feature    5148..5172                     /note="RB"                     /note="right border repeat from nopaline C58 T-DNA"                     /note="color: #ffe4c4"     rep_origin      complement(5263..5851)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"                     /note="color: #ffff00"     CDS             complement(6022..6837)                     /codon_start=1                     /gene="aph(3')-Ia"                     /product="aminoglycoside phosphotransferase"                     /note="KanR"                     /note="confers resistance to kanamycin in bacteria or G418                      (Geneticin(R)) in eukaryotes"                     /note="color: #ccffcc"                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"ORIGIN        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt ccgaacccca      961 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg     1021 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc     1081 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca     1141 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc     1201 ctgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc     1261 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc     1321 catccgagta cgtcctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc     1381 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg     1441 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct     1501 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca     1561 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac     1621 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat     1681 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc     1741 gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat gagactctaa     1801 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc     1861 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc     1921 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt     1981 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc     2041 ttaattctca tgtatcgata acattaacgt ttacaatttc gcgccattcg ccattcaggc     2101 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga     2161 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac     2221 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggtaccg     2281 ggccccccct cgaggtcgac ggtatcgata agcttgatat cgaattcctg cagcccgggg     2341 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag     2401 tgagggttaa ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     2461 tatccgctca caattccaca caacatacga gccggaaghc ataaagtgta aagcctgggg     2521 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc     2581 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     2641 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     2701 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     2761 taacgcagga aagaacatga aggatcgatc tggattttag tactggattt tggttttagg     2821 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt     2881 atatgctcaa cacatgagcg aaaccctata agaaccctaa tttcccttat cgggaaacta     2941 ctcacacatt atttatggag aaaatagaga gagatagatt tgtagagaga gactggtgat     3001 ttcagcgtac cgaattaatt ctctagctag agtattacaa tagctaagaa tttcgtcatc     3061 gctgaataca gttacatttt acaatttgga ctttccgccc ttcttggcct ttatgaggat     3121 ctctctgatt tttcttgcgt cgagttttcc ggtaagacct ttcggtactt cgtccacaaa     3181 cacaactcct ccgcgcaact ttttggcggt tgttacttga ctggcgacgt aatccacgat     3241 ctctttttcc gtcatcgtct ttccgtgctc caaaacaaca acggcggcgg gaagttcacc     3301 ggcgtcatcg tcgggaagac ctgccacgcc cgcgtcgaag atgttggggt gttgtaacaa     3361 tatcgactcc aattcagcgg gggccacctg gtatcctttg tatttaatta aagacttcaa     3421 gcggtcaact atgaagaagt gttcgtcttc gtcccagtaa gctatgtctc cagaatgtag     3481 ccatccatcc ttgtcaatca aggcgttggt cgcttccgga ttgtttacat aaccggacat     3541 aatcataggt cctctgacac ataattcgcc tctctgatta acgcccagcg ttttcccggt     3601 atccagatcc acaaccttcg cttcaaaaaa tggaacaact ttaccgaccg cgcccggttt     3661 atcatccccc tcgggtgtaa tcagaatagc tgatgtagtc tcagtgagcc catatccttg     3721 tcgtatccct ggaagatgga agcgttttgc aaccgcttcc ccgacttctt tcgaaagagg     3781 tgcgccccca gaagcaattt cgtgtaaatt agataaatcg tatttgtcaa tcagagtgct     3841 tttggcgaag aatgaaaata gggttggtac tagcaacgca ctttgaattt tgtaatcctg     3901 aagggatcgt aaaaacagct cttcttcaaa tctatacatt aagacgactc gaaatccaca     3961 tatcaaatat ccgagtgtag taaacattcc aaaaccgtga tggaatggaa caacacttaa     4021 aatcgcagta tccggaatga tttgattgcc aaaaatagga tctctggcat gcgagaatct     4081 gacgcaggca gttctatgcg gaagggccac acccttaggt aacccagtag atccagagga     4141 gttcattatc agtgcaattg ttttgtcacg atcaaaggac tctggtacaa aatcgtattc     4201 attaaaaccg ggaggtagat gagatgtgac gaacgtgtac atcgactgaa atccctggta     4261 atccgtttta gaatccatga taataatttt ctggattatt ggtaattttt tttgcacgtt     4321 caaaattttt tgcaacccct ttttggaaac aaacactacg gtaggctgcg aaatgttcat     4381 actgttgagc aattcacgtt cattataaat gtcgttcgcg ggcgcaactg caactccgat     4441 aaataacgcg cccaacaccg gcataaagaa ttgaagagag ttttcactgc atacgacgat     4501 tctgtgattt gtattcagcc catatcgttt catagcttct gccaaccgaa cggacatttc     4561 gaagtattcc gcgtacgtga tgttcacctc gatatgtgca tctgtaaaag caattgttcc     4621 aggaaccagg gcgtatctct tcatagcctt atgcagttgc tctccagcgg ttccatcctc     4681 tagcggatag aatggcgccg ggcctttctt tatgtttttg gcgtcttcca tgagcttggg     4741 ctgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt     4801 gggattgtgc gtcatccctt acgtcagtgg agatgtcaca tcaatccact tgctttgaag     4861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg     4921 gaccactgtc ggcagaggca tcttgaatga tagcctttcc tttatcgcaa tgatggcatt     4981 tgtaggagcc accttccttt tctactgtcc tttcgatgaa gtgacagata gctgggcaat     5041 ggaatccgag gaggtttccc gaaattaccc tttgttgaaa agtctcaata gccctttggt     5101 cttctgagac tgtatctttg acatttttgg agtaggggta cgatccttga caggatatat     5161 tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat ctcatgtgag caaaaggcca     5221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     5281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     5341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     5401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     5461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     5521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     5581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     5641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag