pCMV-VSVG载体

LOCUS       pCMV-VSVG	6363 bp 	DNA     circular	SYNDEFINITION  pCMV-VSVGACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..6363                     /organism="pCMV-VSVG"                     /mol_type="other DNA"     promoter        84..660                     /label="CMV_immearly_promoter"     misc_feature    148..974                     /label="5_LTR"     misc_feature    163..450                     /label="CAG_enhancer"     misc_feature    617..637                     /label="CMV_fwd_primer"     promoter        618..687                     /label="CMV_promoter"     promoter        630..749                     /label="CMV2_promoter"     misc_feature    661..680                     /label="pCEP_fwd_primer"     misc_feature    663..687                     /label="LNCX_primer"     misc_feature    778..1350                     /label="bGlob_int"     CDS             1276..2955                     /label="ORF frame 1"     gene            1420..2955                     /label="vsv-G"                     /gene="vsv-G"     misc_feature    2920..2952                     /label="VSVG Tag"     terminator      2974..3500                     /label="rb_glob_PA_terminator"     misc_feature    complement(3545..3567)                     /label="M13_pUC_rev_primer"     promoter        complement(3581..3610)                     /label="lac_promoter"     gene            complement(4693..5553)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(4693..5553)                     /label="ORF frame 1"     promoter        complement(5595..5623)                     /label="AmpR_promoter"     misc_feature    complement(6140..6295)                     /label="lacZ_a"     misc_feature    6266..6288                     /label="M13_pUC_fwd_primer"     promoter        6281..6297                     /label="M13_forward20_primer"     promoter        6307..6325                     /label="T7_promoter"ORIGIN    1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca   61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt  121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat  181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt  241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa  301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc  361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct  421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag  481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt  541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac  601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc  661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc  721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg  781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga  841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga  901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt  961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt 1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt 1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata 1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt 1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga 1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac 1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg 1381 tctcatcatt ttggcaaaga attcctcgac ggatccgcca tgaagtgcct tttgtactta 1441 gcctttttat tcattggggt gaattgcaag ttcaccatag tttttccaca caaccaaaaa 1501 ggaaactgga aaaatgttcc ttctaattac cattattgcc cgtcaagctc agatttaaat 1561 tggcataatg acttaatagg cacagcctta caagtcaaaa tgcccaagag tcacaaggct 1621 attcaagcag acggttggat gtgtcatgct tccaaatggg tcactacttg tgatttccgc 1681 tggtatggac cgaagtatat aacacattcc atccgatcct tcactccatc tgtagaacaa 1741 tgcaaggaaa gcattgaaca aacgaaacaa ggaacttggc tgaatccagg cttccctcct 1801 caaagttgtg gatatgcaac tgtgacggat gccgaagcag tgattgtcca ggtgactcct 1861 caccatgtgc tggttgatga atacacagga gaatgggttg attcacagtt catcaacgga 1921 aaatgcagca attacatatg ccccactgtc cataactcta caacctggca ttctgactat 1981 aaggtcaaag ggctatgtga ttctaacctc atttccatgg acatcacctt cttctcagag 2041 gacggagagc tatcatccct gggaaaggag ggcacagggt tcagaagtaa ctactttgct 2101 tatgaaactg gaggcaaggc ctgcaaaatg caatactgca agcattgggg agtcagactc 2161 ccatcaggtg tctggttcga gatggctgat aaggatctct ttgctgcagc cagattccct 2221 gaatgcccag aagggtcaag tatctctgct ccatctcaga cctcagtgga tgtaagtcta 2281 attcaggacg ttgagaggat cttggattat tccctctgcc aagaaacctg gagcaaaatc 2341 agagcgggtc ttccaatctc tccagtggat ctcagctatc ttgctcctaa aaacccagga 2401 accggtcctg ctttcaccat aatcaatggt accctaaaat actttgagac cagatacatc 2461 agagtcgata ttgctgctcc aatcctctca agaatggtcg gaatgatcag tggaactacc 2521 acagaaaggg aactgtggga tgactgggca ccatatgaag acgtggaaat tggacccaat 2581 ggagttctga ggaccagttc aggatataag tttcctttat acatgattgg acatggtatg 2641 ttggactccg atcttcatct tagctcaaag gctcaggtgt tcgaacatcc tcacattcaa 2701 gacgctgctt cgcaacttcc tgatgatgag agtttatttt ttggtgatac tgggctatcc 2761 aaaaatccaa tcgagcttgt agaaggttgg ttcagtagtt ggaaaagctc tattgcctct 2821 tttttcttta tcatagggtt aatcattgga ctattcttgg ttctccgagt tggtatccat 2881 ctttgcatta aattaaagca caccaagaaa agacagattt atacagacat agagatgaac 2941 cgacttggaa agtgataagg atccgtcgag gaattcactc ctcaggtgca ggctgcctat 3001 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt 3061 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa 3121 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga 3181 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg 3241 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag 3301 tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt 3361 tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc 3421 ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc 3481 cctcttctct tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct 3541 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 3601 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 3661 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 3721 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 3781 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 3841 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 3901 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 3961 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 4021 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 4081 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 4141 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 4201 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 4261 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 4321 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 4381 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4441 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4501 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4561 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 4621 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 4681 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 4741 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 4801 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 4861 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 4921 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 4981 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 5041 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 5101 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 5161 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 5221 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 5281 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 5341 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 5401 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 5461 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 5521 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 5581 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 5641 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat 5701 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 5761 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 5821 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 5881 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 5941 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 6001 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 6061 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 6121 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 6181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 6241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 6301 cgcgcgtaat acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct 6361 aga