pLVX-Hom-Nuc1载体

LOCUS       pLVX-Hom-Nuc1           8499 bp ds-DNA    circular SYN 30-JUL-2013COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.comKEYWORDS    pLVX-Hom-Nuc1 VectorSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 8499)  AUTHORS   Clontech Laboratories Inc.FEATURES             Location/Qualifiers     source          1..8499                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     LTR             1..634                     /label="5' LTR"     misc_feature    681..806                     /label="Packaging signal"     misc_feature    1303..1536                     /label="RRE"     misc_feature    2028..2144                     /label="cPPT/CTS"     enhancer        2201..2504                     /label="CMV enhancer"     promoter        2505..2708                     /label="CMV promoter"     misc_feature    2803..2820                     /label="5' MCS"     CDS             2824..2844                     /label="SV40 NLS"     CDS             2854..3174                     /label="DmrB"     misc_feature    3176..3189                     /label="3' MCS"     misc_feature    3202..3775                     /label="IRES"     CDS             3808..4407                     /label="PuroR"     misc_feature    4421..5009                     /label="WPRE"     LTR             5217..5850                     /label="3' LTR"     rep_origin      complement(6381..6966)                     /direction=LEFT                     /label="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(7137..7997)                     /label="AmpR"     promoter        complement(7998..8102)                     /label="AmpR promoter"ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt     2821 atgcctaaga agaagagaaa ggtggcttct agaggagtgc aggtggagac tatctcccca     2881 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg     2941 cttgaagatg gaaagaaagt tgattcctcc cgggacagaa acaagccctt taagtttatg     3001 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt     3061 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc     3121 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaaagcggc     3181 cgcggatcct acgtatgacc gcccctctcc ctcccccccc cctaacgtta ctggccgaag     3241 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc     3301 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg     3361 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc     3421 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc     3481 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa     3541 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct     3601 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg     3661 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg     3721 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg     3781 ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc     3841 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc     3901 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa     3961 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc     4021 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc     4081 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc     4141 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg     4201 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc     4261 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac     4321 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg     4381 tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac aatcaacctc tggattacaa     4441 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata     4501 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc     4561 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg     4621 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac     4681 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat     4741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt     4801 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat     4861 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc     4921 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag     4981 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa     5041 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta     5101 gaagcacaag aggaggagga ggtgggtttt tccagtcaca cctcaggtac ctttaagacc     5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga     5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca     5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac     5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa     5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag     5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct     5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg     5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg     5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct     5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag     5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac     5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt     5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt     5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga     6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     6901 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga     6961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca     7021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat     7081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac     7141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt     7201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt     7261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag     7321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct     7381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt     7441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc     7501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt     7561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg     7621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg     7681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct     7741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc     7801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt     7861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt     7921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg     7981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat     8041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg     8101 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat     8161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     8221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg     8281 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat     8341 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat     8401 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag     8461 aaattgtatt tgttaaatat gtactacaaa cttagtagt