pLVX-mCherry-N1载体

LOCUS       pLVX-mCherry-N1   8778 bp    DNA   circular             7-OCT-2009COMMENT     Created by Clontech Laboratories Inc.COMMENT     Cat. No. 632562COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     LTR             1..635                     /label=5'\LTR     misc_feature    685..822                     /label=packaging\signal     misc_feature    4746..5337                     /label=WPRE     CDS             complement(7464..8460)                     /label=Amp(R)     misc_binding    1303..1536                     /label=RRE     misc_binding    636..653                     /label=PBS     LTR             5540..6176                     /label=3'\LTR     promoter        3604..4112                     /label=PGK\Promoter     CDS             4133..4732                     /label=Puro(R)     CDS             2875..3585                     /label=mCherry     misc_feature    2028..2151                     /label=cPPT/CTS\(DNA\flap)     promoter        2185..2787                     /label=CMV\IE\Promoter     rep_origin      complement(6646..7319)                     /label=pUC\ori     misc_feature    2815..2867                     /label=MCSBASE COUNT     2178 a      2271 c      2335 g      1994 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa      2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt      2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc      2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc      2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca      2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta      2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa      2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc      2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag      2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcatggtg      2881 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac      2941 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc      3001 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc      3061 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc      3121 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg      3181 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag      3241 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag      3301 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg      3361 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc      3421 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc      3481 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc      3541 gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagcggc cgcgactcta      3601 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag      3661 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat      3721 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc      3781 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa      3841 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc      3901 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga      3961 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg      4021 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc      4081 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga      4141 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct      4201 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat      4261 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa      4321 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga      4381 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct      4441 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg      4501 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt      4561 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc      4621 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga      4681 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct      4741 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg      4801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt      4861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg      4921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc      4981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc      5041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc      5101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc      5161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg      5221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc      5281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa      5341 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc      5401 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt      5461 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt      5521 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct      5581 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg      5641 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc      5701 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct      5761 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc      5821 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc      5881 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg      5941 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc      6001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta      6061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact      6121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta      6181 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg      6241 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca      6301 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga      6361 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt      6421 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga      6481 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc      6541 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg      6601 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc      6661 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc      6721 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga      6781 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc      6841 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat      6901 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg      6961 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc      7021 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga      7081 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact      7141 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt      7201 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag      7261 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg      7321 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa      7381 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata      7441 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg      7501 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata      7561 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg      7621 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct      7681 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt      7741 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc      7801 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga      7861 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt      7921 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc      7981 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa      8041 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca      8101 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca      8161 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct      8221 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc      8281 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa      8341 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt      8401 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc      8461 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag      8521 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa      8581 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca      8641 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact      8701 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg      8761 tactacaaac ttagtagt //