Cynomolgus TIGIT Protein, Fc Tag

内毒素:
Less than 1.0 EU per μg by the LAL method.

纯度:
>95% as determined by SDS-PAGE.

活性:
Measured by its binding ability in a functional ELISA against immobilized Human CD155, His Tag (Cat# CD5-H5223, 1 μg/well). The linear range is 0.2-20 μg/mL.

产品背景:
T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

产品描述:
Cynomolgus TIGIT, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Met 89. the reducing (R) protein migrates as 40-50 kDa, and the non-reducing (NR) protein migrates as 80-116 kDa in SDS-PAGE.