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JIM-Antibodies JIM系列抗体 鼠抗单克隆抗体

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  • JIM-Antibodies  JIM系列抗体 鼠抗单克隆抗体

    Antibody Name: JIM13
      Antibody Name: JIM13
     Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
     Catolog#:  
     Isotype: IgM
     Antibody Type: Monoclonal
     Source Animal: Rat
     Detail Info for Purified Antibody:  
     Immunogen: Arabinogalactan protein (AGP2)
     Antigen: Arabinogalactan, Arabinogalactan protein
     Plant Origin: Carrot (Daucus carota)
     Working Concentration: Neat or diluted 1:10
     Status of Antibody: Hybridoma supernatant
     Polysaccharide Cross Reactivity: JIM13.pdf
     Epitope Structure for Carbonhydrate Antigen: (beta)GlcA1->3(alpha)GalA1->2Rha
     M.W. of Protein Antigen(KDa): 80-100
     Protein Cross Reactivity: Arabinogalactan proteins from diverse plants
     References:

    J. P. Knox, P. J. Linstead, J. Peart, C. Cooper, and K. Roberts. (1991)
    Developmentally-regulated 
    epitopes of cell surface arabinogalactan-proteins
    and their relation to root tissue pattern 
    formation. Plant Journal 1:317-326.

    E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant
    cell surface 
    epitopes in exudate gums. Carbohydr.Polym.24:281-286.

    E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie,

     and J. P. Knox. (1996) Characterization of carbohydrate structural 

    features recognized by anti-arabinogalactan-protein monoclonal antibodies.
    Glycobiology 6:131-139.

     Comments:

    JIM13 binds to various exudate gums, including gum arabic and gum ghatti. JIM13 

    also binds to rhamnogalacturonan I from sycamore maple. The oligosaccharide 

    shown above is the most effective oligosaccharide competitor tested to date.

     
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    Detailed description of the Arabidopsis inflorescence stem expansion glycoprofiling:

    Experiment Title:Arabidopsis inflorescence stem expansion glycoprofiling
    Experiment Title: Arabidopsis inflorescence stem expansion glycoprofiling
    Description:

    Series of transverse sections at various regions of a bolting inflorescenc

    e primary stem to capture compositional changes during cell wall development.

    Organism: Arabidopsis thaliana
    Tissues: Inflorescence stem
    Treatments: Developmental stage (young, maximum growth rate, and cessation)
    Design: Glycoprofiling_Arab_inflr_exp-design.pdf
    Antibodies Employed: MAC207
    CCRC-M1
    CCRC-M2
    CCRC-M7
    CCRC-M8
    CCRC-M9
    CCRC-M11
    CCRC-M12
    CCRC-M13
    CCRC-M14
    CCRC-M15
    CCRC-M16
    CCRC-M22
    CCRC-M30
    CCRC-M31
    CCRC-M32
    CCRC-M34
    CCRC-M36
    CCRC-M38
    CCRC-M44
    CCRC-M48
    CCRC-M49
    CCRC-M70
    JIM1
    JIM3
    JIM4
    JIM5
    JIM7
    JIM8
    JIM11
    JIM12
    JIM13
    JIM14
    JIM15
    JIM16
    JIM17
    JIM19
    JIM20
    JIM84
    JIM93
    JIM94
    JIM101
    JIM131
    JIM132
    JIM133
    JIM136
    JIM137
    LM10
    LM18
    LM19
    LM20
    MAC204
    MAC265
    MAC266
    PN 16.4B4
    Immunolableing Protocol: Immunolabeling_protocol_for_CCRC.txt
    Imageing Protocol: Imaging_protocol_for_CCRC.txt
    Contributor Name: Hardy Hall, Jingling Cheung, Brian Ellis
    Contributor Address: Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
    Related Publication: Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding
    Arabidopsis stem (DOI: 10.1111/tpj.12111)
    Publication Status: In Press
    Related Image Sets: CCRC-M1-Panel
    CCRC-M11-panel
    CCRC-M12-panel
    CCRC-M13-panel
    CCRC-M14-panel
    CCRC-M15-panel
    CCRC-M16-panel
    CCRC-M2-panel
    CCRC-M22-panel
    CCRC-M30-panel
    CCRC-M31-panel
    CCRC-M32-panel
    CCRC-M34-panel
    CCRC-M36-panel
    CCRC-M38-panel
    CCRC-M44-panel
    CCRC-M48-panel
    CCRC-M49-panel
    CCRC-M7-panel
    CCRC-M70-panel
    CCRC-M8-panel
    CCRC-M9-panel
    JIM1-panel
    JIM101-panel
    JIM11-panel
    JIM12-panel
    JIM13-panel
    JIM131-panel
    JIM132-panel
    JIM133-panel
    JIM136-panel
    JIM137-panel
    JIM14-panel
    JIM15-panel
    JIM16-panel
    JIM17-panel
    JIM19-panel
    JIM20-panel
    JIM3-panel
    JIM4-panel
    JIM5-panel
    JIM7-panel
    JIM8-panel
    JIM84-panel
    JIM93-panel
    JIM94-panel
    LM10-panel
    LM18-panel
    LM19-panel
    LM20-panel
    MAC204-panel
    MAC207-panel
    MAC265-panel
    MAC266-panel
    PN 16.4B4-panel
     
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