phospho-ATG3 (Tyr18)抗体

 

 phospho-ATG3 (Tyr18) Antibody
 

Cat#：PL0301297
 

Isotype:Rabbit Polyclonal IgG
 

Immunogen：PhosphoPeptide derived from Hu ATG3 around the phosphorylation site of Tyr18.  
 

Purification: Immunogen affinity purified

Storage Buffer: PBS, pH 7.4(1%BSA and 0.1% Sodium azide)

Background：

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Specificity Reaction：Hu, Mo, Rat, Chi, Dog, Pig, Cow, Hor

 

Application Notes:WB: 1:100-500 E: 1:500-1000 IHC: 1:100-500 IF: 1:100-500

 

Store at –20ºC for one year. Avoid repeated freeze-thaw cycles. At 4ºC for 2-3 weeks after reconstituted.

 

 

PLlabs公司可供应相应抗体的生物素、辣根过氧化物酶、荧光素（FITC、PE、  CY3、 CY5、 CY7、 PE- CY3/5/7)标记抗体，欢迎大家咨询订购。