兔抗尿苷二磷酸葡萄糖焦磷酸化酶多克隆抗体

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兔抗尿苷二磷酸葡萄糖焦磷酸化酶多克隆抗体介绍：

货   号：AS05 086

中文名称：兔抗尿苷二磷酸葡萄糖焦磷酸化酶多克隆抗体

英文名称：UGPase|UDP-glucose pyrophosphorylase (cytoplasm marker)

应用：western blot (WB)

规格：200 µl

价格：3975元

 

兔抗尿苷二磷酸葡萄糖焦磷酸化酶多克隆抗体简介：

PRODUCT INFORMATION background   UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9.  is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011).  immunogen   recombinant UGPase Q43772 overexpressed and purified from E.coli host   rabbit clonality   polyclonal purity   serum, format   lyophilized quantity   50 µl reconstitution   For reconstitution add 50 µl of sterile water. storage   store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications   western blot (WB), immunolocalization (IL) related products   AS14 2813 | UDP-glucose pyrophosphorylase (cytoplasm marker) (Hordeum vulgare)  For cytoplasmic marker for Chlamydomonas reinhardtii - please check NAB1 collection of antibodies to carbohydrate metabolism recommended secondary antibody additional information   cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al. (2002)

APPLICATION INFORMATION
recommended dilution		1: 1000 - 1: 3000  with ECL (WB), 1: 1500 (IL)
expected | apparent MW		51.6 kDa
confirmed reactivity		Arabidopsis thaliana, Brassica rapa, Capsicum annuum, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Marchantia polymorpha, M.vaginalis, Nicotiana tabacum, Oryza sativa, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivum
predicted reactivity		Brassica pekinensis, Capsella rubella, Glycine max, Glycine soja, Gossipium hirsutum, Pinus taeda,Populus tremula, Ricinus communis, Saccharum officinarum, Zea mays, Vitis vinifera, Citrus sinensis, Jatropha curcas, Cucumis melo, Bambusa oldhamii, Eucalyptus grandis, Theobroma cacao, Gossypium hirsutum, Sorghum bicolor, Amorpha fruticosa, Brachypodium distachyon, for more species, please inquire
not reactive in		diatoms
additional information		this antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana
selected references		Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8.  Kolb et al. (2015). FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis thaliana. Plant Physiol. 2015 Feb 19. pii: pp.114.253377.  Rounis et al. (2014). Seeded and Parthenocarpic Cherry Tomato Fruits Exhibit Similar Sucrose, Glucose, and Fructose Levels, Despite Dissimilarities in UGPase and SPS Gene Expression and Enzyme Activity. J. Plant Growth Regul., July 2014. (immunolocalization) Komatsu et al. (2014). Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the Liverwort Marchantia polymorpha L. 1. Plant Physiol. 2014 Sep;166(1):411-27. doi: 10.1104/pp.114.245100. Epub 2014 Aug 5.  Fukayama et al. (2014). Nocturnal phosphorylation of phosphoenolpyruvate carboxylase in the leaves of hygrophytic C3 monocots. Bioscience, Biotechnology and Biochemistry.  Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7.

 

 A 1-year-old greehouse grown plant was dissectedinto different tissues, which were then used for enzyme assays and immunoblot analyses. Equal amounts of total protein (7.5 μg) were loaded on each lane. SDS-PAGE was run on a 7.5% gel. Immunoblot was done using Amersham PVDF transfer membrane. Primary antibodies against barley UGPase were used in 1: 1000 dilution. Secondary antibodies (Amersham ECL Rabbit IgG, HRP-Linked Whole Antibody from donkey) were used at 1:10 000.

ff - female flower, mf - male flower, yl - young leaf, ml - mature leaf, sbk - stem bark, sph - stem phloem and cambium, sxy - stem xylem, rxy - root xylem

 

15 µg of  total soluble protein  extract from leaves and stems of  Solanum tuberosum (1),  Solanum sogarandinum (2),   Lycopersicum esculentum (3),  Lycopersicum chilense  (4) , Arabidopsis thaliana (5) , Cucumis sativus (6) ,  Festuca arundinacea  (7) , Nicotiana tabacum (8) and Capsicum annuum (9)  were separated  on 10% SDS-PAGE and blotted onto nitrocellulose .  After blocking with 5% milk in TBST , blots were incubated with the primary antibody at a dilution of 1:1500 in TBST for 1h  at room temperature. Following incubation and wash steps, blots were incubated withSIGMA secondary Anti-Rabbit IgG , Alkaline Phosphatase Conjugat