产品库
登录
首页 找仪器 社区 百科 新品 供应商 品牌 应用 资料 仪企号 展会 标准 求购 招中标

二抗及链霉亲和素

产品信息
  •       免疫荧光技术是将免疫学方法(抗原抗体特异结合)与荧光标记技术结合起来研究特异蛋白抗原在细胞内分布的方法。由于荧光素所发的荧光可在荧光显微镜下检出，从而可对抗原进行细胞定位。
        免疫荧光检测方法根据待检测抗原的丰度采用不同的荧光标记策略。对于高丰度的抗原，可采用荧光标记一抗直接检测的方式；对于中等丰度的抗原，可采用荧光标记二抗的方式对待检测的抗原进行信号放大；对于低等丰度的抗原，可采用生物素标记抗体和荧光标记链霉亲和素的方式对待检测的抗原进行二级信号放大。在整个免疫荧光检测的过程中，荧光染料的性能（信号强度，稳定性，背景信号）对免疫荧光检测的结果发挥着关键性的作用。早期较常用的FITC荧光素标记抗体由于其信号弱，稳定性差，目前正逐渐被新型荧光染料（如Alexa Fluor, Dylight）所取代。
        Applied BioProbes公司ZX推出了一系列性能优良的Andy Fluor荧光染料。这些新型荧光染料跟传统的荧光染料（如FITC）相比，具有无可比拟的优越性。公司新推出了一系列Andy Fluor和生物素标记的二抗及相应的标记服务，为广大科研用户提供了广阔的选择。


    产品特点:
    • 荧光信号强；
    • 背景信号低；
    • 光稳定性好；
    • 多色荧光可供选择；
    • 仪器兼容性好；


    荧光标记二抗及链霉亲和素选购指南
     
    Secondary Antibody Streptavidin
    Andy Fluor™ Dyes Other Fluorescent Labels Biotin & HRP Labels Andy Fluor™ & Cy® Dyes
    Andy Fluor™ 350 Cy 3 Biotin Andy Fluor™ 350
    Andy Fluor™ 405 Cy 5 HRP Andy Fluor™ 488
    Andy Fluor™ 430 Cy 5.5   Andy Fluor™ 555
    Andy Fluor™ 488 Cy 7   Andy Fluor™ 594
    Andy Fluor™ 555 FITC   Andy Fluor™ 647
    Andy Fluor™ 568     Cy®3
    Andy Fluor™ 594     Cy®5
    Andy Fluor™ 647      
    Andy Fluor™ 680      
    Andy Fluor™ 750      


    Andy Fluor™ dye conjugates have brighter fluorescence than other fluorophores.


    Figure 1. Comparison of relative fluorescence of goat anti-rabbit IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, Cy®3, and Cy®5.



    Figure 2. Flow cytometry comparison of the brightness of goat anti-mouse IgG antibody conjugates prepared from Andy Fluor™ 488, 555, and 647 with Cy®2, DyLight™ 488, Cy®3, DyLight™ 550, and DyLight™ 650.


    Andy Fluor™ dye conjugates have better photostability than other fluorophores.



    Figure 3. Comparison of the photobleaching rates of Andy Fluor™ 488 goat anti-mouse IgG (H+L) (L109B) with FITC goat anti-mouse IgG (H+L) (L146B). The cytoskeleton of HeLa cells was labeled with mouse monoclonal anti-α-tubulin antibody in combination with Andy Fluor™ 488 goat anti-mouse IgG (H+L) antibody (top series) or with mouse monoclonal anti-α-tubulin antibody in combination with FITC goat anti-mouse IgG (H+L) antibody (bottom series). The fluorescence imaging was taken at 60-second intervals (0, 60, and 120 seconds of exposure).



    Figure 4. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, and then visualized with goat anti-mouse IgG antibodies conjugated with either Andy Fluor™ 488 (top, left), Andy Fluor™ 555 (top, right), Andy Fluor™ 568 (bottom, left), or Andy Fluor™ 647 (bottom, right). Nuclei are counterstained with DAPI (blue).



    Figure 5. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with green-fluorescent Andy Fluor™ 488 goat anti-rabbit IgG antibody (green). Nuclei were counterstained with DAPI (blue).



    Figure 6. Immunofluorescent stain of CCSP in mouse lung tissue. FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, and then visualized with either Andy Fluor™ 555 (red, left), or Andy Fluor™ 568 (red, right). Nuclei were counterstained with DAPI (blue).



    Figure 7. Immunofluorescent stain of α-tubulin in BEAS2BNNK cells and CCSP in mouse lung tissue. α-Tubulin in fixed and permeabilized BEAS2BNNK cells was labeled with anti-α-tubulin primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, left). FFPE samples of mouse lung were labeled with rabbit anti-CCSP primary antibody, followed by incubation with biotin goat anti-mouse IgG antibody, and then visualized with Andy Fluor™ 488 Streptavidin (green, middle and right). Nuclei were counterstained with DAPI (blue).
     
    温馨提示：不可用于临床ZL。
    • 信息声明：本产品供应信息由仪器网为您整合，供应商为（上海伯易生物科技有限公司），内容包括 （二抗及链霉亲和素）的品牌、型号、技术参数、详细介绍等；如果您想了解更多关于 （二抗及链霉亲和素）的信息，请直接联系供应商，给供应商留言！
    供应商产品推荐
      您可能感兴趣的产品