培养条件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1)无菌室及无菌操作台(laminarflow)用紫外灯照射30-60分钟灭菌,70%ethanol擦拭无菌操作抬面,并开启无菌操作台风扇运转10分钟。2)细胞用的培养基如有相同切记不可共享培养基,以避免失误混淆或细胞间污染。 (注:实验操作应在抬面之ZY无菌区域,勿在边缘之非无菌区域操作。)培养细胞将复合细胞计数要求的细胞悬液分装入培养瓶内,将培养瓶放入37℃和5%CO2的培养箱内2-4小时(或者24-48小时)后换液继续培养培养,换液的时间由细胞情况而定。
Naringin dihydrochale 柚皮苷二氢查尔酮 18916-17-1 20mg
Ginkgolide A 银杏内酯A 15291-75-5 20mg
Betulinaldehyde 白桦脂醛 13159-28-9 20mg
α-Terpineol α-松油醇 10482-56-1 20mg
2,4(1H,3H)-Quinazolinedione 亚苯甲酰基脲 86-96-4 20mg
透析袋 扁宽45 截留分子量10000 0.5m/即用型 管
15ml离心管(尖底罗口) 100支 包
Meloxicam 美洛昔康 5g 瓶
Salicylic acid 水杨酸 250g 瓶
CM Cellulose 羧甲基纤维素钠 25g 瓶
Lentinan 香菇多糖 1g 瓶
卢戈碘液(标准碘液) 100ml 瓶
Aseorbie acid 抗坏血酸 (Vitamin C) 25g 瓶NSC 348884 N1,
Clozapine N-oxide 8-chloro-11-(4-methyl-4-oxido-1-erazinyl)-5H-dibenzo[b,e][1,4]diazepine
Furafylline (1 mg) 3-(2-furanylmethyl)-3,9-dihydro-1,8-dimethyl-1H-purine-2,6-dione
L-Propio
nylcarnitine (chloride) (100 mg) Dromos|Levocarnitine propio
nate|ST 261; 3-carboxy-N,N,N-trimethyl-2R-(1-oxopropoxy)-1-propanaminium, monochloride
Trimethoprim-PEG-amine (trifluoroacetate salt) (5 mg) N-(2-(2-(2-(2-aminoethoxy)ethoxy)ethoxy)ethyl)-2-(4-((2,6-diaminopyrimidin-4-yl)methyl)-2,6-dimethoxyphenoxy)acetamide tris(2,2,2-trifluoroacetate)
PPQ-102 (5 mg) 6,7-dihydro-7,9-dimethyl-6-(5-methyl-2-furanyl)-11-phenyl-Pyrimido[4’,5’:3,4]pyrrolo[1,2-a]quinoxaline-8,10(5H,9)-dione
生物素标记三甲基化组蛋白H3K9多肽 Biotinylated Trimethyl Histone H3K9 Peptide (200 ul)
Ac-LEVD-AFC, Caspase-4荧光底物 Caspase-4 Substrate LEVD-AFC
Entinostat, SNDX-275 MS-275 (Entinostat, SNDX-275)
1,4-Dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo(4,5-d)pyrimidin-7-one; M Zaprinast
人肥胖YZ素 Obestatin (Human)
3’-[α-Amino-p-methoxyhydrocinnamamido]-3’-deoxy-N,N-dimethyladenosine dihydrochloride Puromycin Dihydrochloride
(9β,13α,14β,20α)-3-Hydroxy-9,13-dimethyl-2-oxo-24,25,26-trinoroleana-1(10),3,5,7-tetraen-29-oic acid Celastrol
人黑色素瘤细胞;A875价格8- Bromoadenosine- 3’, 5’- cyclic monophosphate; cyclic 8- bromoadenosine- 3’, 5’- monophosphate; 8- bromoadenosine- 3’, 5’-Mo
nophosphate 8-Br-cAMP
(5Z)-2-((Thiophen-2-yl)methylamino)-5-((quinolin-6-yl)methylene)thiazol-4(5H)-one RO-3306
3-(3-(4-Fluorophenyl)-5-oxo-4,5-dihydro-[1,2,4]triazolo[4,3-a]quinazolin-1-yl-N-(3-methylphenethyl)propanamide Smo Antagonist, SA9
Taxotere; Docefrez Docetaxel
人黑色素瘤细胞;A875价格操作步骤:
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。