Recombinant SARS Associated Coronavirus MatrixIntroduction SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Recombinant SARS Associated Coronavirus MatrixDescription The E.Coli derived 30kDa recombinant protein contains the Matrix protein 182-216 amino acids immunodominant regions.
Purification Method SARS Matrix protein was purified by proprietary chromatographic technique.
Purity SARS Matrix Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Recombinant SARS Associated Coronavirus MatrixFormulation 50mM Tris-HCl, 60mM NaCl, and 50% glycerol.
Storage SARS Matrix protein is shipped at ambient temperature.
Upon arrival, store at -200C.
Stability Five year frozen, 6 month at +40C.
Recombinant SARS Associated Coronavirus MatrixSpecificity SARS Matrix protein is Immunoreactive with sera of SARS-infected individuals.
Applications SARS Matrix antigen can be used for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.
Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
