培养条件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 10%FBS
1)无菌室及无菌操作台(laminarflow)用紫外灯照射30-60分钟灭菌,70%ethanol擦拭无菌操作抬面,并开启无菌操作台风扇运转10分钟。2)细胞用的培养基如有相同切记不可共享培养基,以避免失误混淆或细胞间污染。 (注:实验操作应在抬面之ZY无菌区域,勿在边缘之非无菌区域操作。)培养细胞将复合细胞计数要求的细胞悬液分装入培养瓶内,将培养瓶放入37℃和5%CO2的培养箱内2-4小时(或者24-48小时)后换液继续培养培养,换液的时间由细胞情况而定。
DEA No
nOate (50 mg) Diethylammo
nium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate; Diethylamine No
nOate|DEA/NO; DEA NONOate
γ-CEHC (5 mg) 3,4-dihydro-6-hydroxy-2,7,8-trimethyl-2H-1-benzopyran-2-propanoic acid; 2,7,8-trimethyl-2-(β-Carboxy-Ethyl)-6-Hydroxychroman|GTM|γ-Tocopherol me
tabolite; γ-CEHC
Rimo
nabant (10 mg) 5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-N-1-peridinyl-1H-pyrazole-3-carboxamide; SR141716; Rimonabant
JWH 081 N-(5-hydroxypentyl) me
tabolite (10 mg) (1-(5-hydroxypentyl)-1H-indol-3-yl)(4-methoxynaphthalen-1-yl)methanone; JWH 081 N-(5-hydroxypentyl) me
tabolite
JWH 018 N-(1-methylbutyl) isomer (1 mg) naphthalen-1-yl(1-(pentan-2-yl)-1H-indol-3-yl)methanone; JWH 073 1-methylbutyl homolog; JWH 018 N-(1-methylbutyl) isomer
2,3-Methylenedioxy Pyrovalerone (hydrochloride) (5 mg) 1-(benzo[d][1,3]dioxol-4-yl)-2-(peridin-1-yl)pentan-1-one, monohydrochloride; 2,3-MDPV; 2,3-Methylenedioxy Pyrovalerone (hydrochloride)
N,N-Dimethylcathinone (hydrochloride) (50 mg) 2-(dimethylamino)-1-phenyl-1-propanone, monohydrochloride; me
tamfepramone; N,N-Dimethylcathinone (hydrochloride)
HU-210 (10 mg) 3-(1,1’-dimethylheptyl)-6aR,7,10,10aR-tetrahydro-1-hydroxy-6,6-dimethyl-6H-dibenzo[b,d]pyran-9-methanol; HU-210
Docosahexaenoic Acid (500 mg) 4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoic acid; Cervo
nic Acid|DHA; Docosahexaenoic Acid
AMD 3465 (hydrobromide) (5 mg) N-[[4-(1,4,8,11-tetraazacyclotetradec-1-ylmethyl)phenyl]methyl]-2-pyridinemethanamine, hexahydrobromide; AMD 3465 (hydrobromide)
Tandutinib (25 mg) 4-[6-methoxy-7-[3-(1-peridinyl)propoxy]-4-quinazolinyl]-N-[4-(1-methylethoxy)phenyl]-1-perazinecarboxamide; CT 53518|MLN 518; Tandutinib
(S)-nitro-Blebbistatin (25 mg) (3aS)-1,2,3,3a-tetrahydro-3a-hydroxy-7-nitro-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one; S-(-)-7-Desmethyl-8-nitro Blebbistatin; (S)-nitro-Blebbistatin
STK393606 (25 mg) 2-[[(6-bromo-3H-imidazo[4,5-b]pyridin-2-yl)thio]methyl]-benzonitrile; STK393606
Daunorubicin (hydrochloride) (100 mg) 8S-acetyl-10S-[(3-amino-2,3,6-trideoxy-a-L-lyxo-hexopyranosyl)oxy]-7,8,9,10-tetrahydro-6,8,11-trihydroxy-1-methoxy-5,12-naphthacenedione, monohydrochloride; NDC 0082-4155|o
ndena|RP 13057; Daunorubicin (hydrochloride)
Wogo
nin (5 mg) 5,7-dihydroxy-8-methoxy-2-phenyl-4H-1-benzopyran-4-one; BRN 0287152; Wogonin
Phenylmethylsulfo
nyl fluoride (25 g) benzenemethanesulfo
nyl fluoride; PMSF|NSC 88499|Benzylsulfo
nyl fluoride; Phenylmethylsulfo
nyl fluoride
Efavirenz (50 mg) (4S)-6-chloro-4-(2-cyclopropylethynyl)-1,4-dihydro-4-(trifluoromethyl)-2H-3,1-benzoxazin-2-one; DMP 266|EFV|L-743,726; Efavirenz
UR- N-(2-hydroxypentyl) me
tabolite (10 mg) (1-(2-hydroxypentyl)-1H-indol-3-yl)(2,2,3,3-tetramethylcyclopropyl)methanone; UR- N-(2-hydroxypentyl) me
tabolite
BB-22 6-hydroxyisoquinoline isomer (1 mg) isoquinolin-6-yl 1-(cyclohexylmethyl)-1H-indole-3-carboxylate; BB-22 6-hydroxyisoquinoline isomer
7-dehydro Cholesterol (50 g) cholesta-5,7-dien-3β-ol; 7-DHC|NSC 18159|Provitamin D3|Δ7-Cholesterol; 7-dehydro Cholesterol
TAPI-0 (5 mg) N-[2-[2-(hydroxyamino)-2-oxoethyl]-4-methyl-1-oxopentyl]-3-(2-naphthalenyl)-L-alanyl-L-alaninamide; TNF Protease Inhibitor TAPI-0胰凝蛋白酶(含10mL酶解缓冲液) 1mL
结晶紫中性红胆盐葡萄糖琼脂(VRBGA) 规格: 250g 用途: 用于阪崎肠杆菌的选择性分离培养以及肠道菌的计数和鉴别(SN/T 1632.1-2005)。
肌肌酸激酶(CKM)天然蛋白 英文名称: Native Creatine Kinase, Muscle (CKM)
叉框蛋白O3(FOXO3)重组蛋白 英文名称: Recombinant Forkhead Box Protein O3 (FOXO3)
基质金属蛋白酶9(MMP9)重组蛋白 英文名称: Recombinant Matrix me
talloproteinase 9 (MMP9)
神经元正五聚蛋白Ⅱ(NPTX2)重组蛋白 英文名称: Recombinant Neuro
nal Pentraxin II (NPTX2)
粘蛋白17(MUC17)重组蛋白 英文名称: Recombinant Mucin 17 (MUC17)
J82(人膀胱细胞) 5×106cells/瓶×2
VE(人血管内皮细胞) 5×106cells/瓶×2
人胶质母细胞瘤细胞 英文名称: A172
人食管细胞 英文名称: KYSE410
小鼠肺细胞 英文名称: Lewis
无菌豚鼠血清/Sterile Defidrinated Guinea pig Blood BR 200毫升 国产/进口
硫柠胆蔗琼脂培养基/TCBS琼脂/TCBS Agar 用于霍乱、副溶弧菌的分离培养 250克 国产/进口
犬细支气管上皮细胞;CBE1价格坂崎杆菌显色培养基/Enterobacter Sakazakii Chromogenic Medium 用于坂崎杆菌的显色培养,坂崎杆菌显蓝色,其他肠杆菌显无色 1升 国产/进口
BL琼脂培养基/BL Medium 双岐杆菌分离培养 250克 国产/进口
人皮肤肥大细胞完全培养基 100mL
小鼠肠平滑肌细胞完全培养基 100mL
胆硫琼脂培养基(DHL) 规格: 250g 用途: 用于肠道致病菌特别是沙门氏菌和志贺氏菌的选择性分离培养(GB 4789.4-2010和SN/T2206.1-2008)。
脑—心浸萃液态培养基 规格: 100g 用途: 广泛用于霉菌、酵母、细菌的培养,括营养要求较高的微生物的培养,特别用于饮用天然矿泉水和城...
X-射线修复交叉互补蛋白6(XRCC6)重组蛋白 英文名称: Recombinant X-Ray Repair Cross Complementing 6 (XRCC6)
犬细支气管上皮细胞;CBE1价格操作步骤:
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。