Recombinant Human Non-Muscle Myosin-II Regulatory Light Chain

Synonyms Non-Muscle Myosin-II Regulatory Light Chain, NMM.

Introduction Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca +2 Calmodulin regulated light chain kinase (MLCK).

Recombinant Human Non-Muscle Myosin-II Regulatory Light ChainDescription Non-Muscle Myosin-II Regulatory Light Chain Human Recombinant full length expressed in E.coli.

The NMM is purified by proprietary chromatographic techniques.

Source Escherichia Coli.

Physical Appearance Sterile Filtered clear solution.

Formulation NMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.

Recombinant Human Non-Muscle Myosin-II Regulatory Light ChainStability Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.

Please prevent freeze-thaw cycles.

Purity Greater than 95.0% as determined by SDS-PAGE.

Activity Assayed for phosphorilation by MLCK.

Recombinant Human Non-Muscle Myosin-II Regulatory Light ChainAmino Acid Sequence MSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD.

Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.