Recombinant Human Phosphoinositide 3-kinase beta P110β/p85a

Synonyms  Phosphoinositide 3-kinase beta p110?/p85?, PI3K ?, PI3Kb. 

Introduction The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of

PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2).

It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr,

HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis.

PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line.

It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors. 

Recombinant Human Phosphoinositide 3-kinase beta P110β/p85aDescription  Phosphoinositide 3-kinase beta Human Recombinant is a glycosilated protein having a molecular weight as follows: p85? chain 83.5 kDa, p110? chain 124.3 kDa. 

Source  Sf9 insect cells. 

Physical Appearance Sterile filtered liquid formualtion. 

Formulation 0.9 mg/ml solution in PBS and 2mM MgCl2. 

Stability PI3Kb although stable at 4°C for 1 week, should be stored desiccated below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles. 

Recombinant Human Phosphoinositide 3-kinase beta P110β/p85aPurity  Greater than 90.0% as determined by SDS Page. 

Biological Activity  The specific activity was found to be 3,000 units/mg (1 unit is defined as 1 picomole phosphate transferred to PIP2 per minute).

Recombinant Human Phosphoinositide 3-kinase beta P110β/p85aUsage  CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.