| Description |  | Monoclonal Antibody against Nuclear Pore Complex Proteins, NUP 153 |
| Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | | QE5 |
| Form | | Purified Antibody (Stored in PBS + 0.03% Thimerosal) |
| Host | | Mouse |
| Species Reactivity | | NUP 153 Nuclear Pore Complex Protein Monoclonal AntibodyEukaryote |
| IsoType | | IgG1 |
| [Ab] | | 1 mg/mL |
| Specificity | | The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins. It recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern. |
| Uses | | This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). |
| Suggested Working Dilution | | NUP 153 Nuclear Pore Complex Protein Monoclonal AntibodyThe optimal working dilution should be determined for each specific assay condition.
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| Storage | | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | | C Smythe, HE Jenkins, CJ Hutchison. Incorporation of the nuclear pore basket protein nup153 into nuclear pore structures is dependent upon lamina assembly: evidence from cell-free extracts of Xenopus eggs. EMBO J 19(15):3918-3931, 2000. N Panté, R Bastos, I McMorrow, B Burke, U Aebi. Interactions and three dimensional localization of a group of nuclear pore complex proteins. J Cell Biol 126:603-617, 1994. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
