| Description |  | Purified Monoclonal Antibody against Nuclear Pore Complex Proteins |
| Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | | MAb414 |
| Form | | Purified Antibody (Stored in PBS + 0.03% Thimerosal) |
| Host | | Mouse |
| Species Reactivity | | Nuclear Pore Complex Proteins (MAb414) Monoclonal Antibody, Purified Vertebrate, Xenopus, Yeast |
| IsoType | | IgG1 |
| [Ab] | | 1 mg/mL |
| Specificity | | MAb414 is a reliable general purpose monoclonal antibody which recognizes a related family of NPC proteins. This antibody is ideal for studying the morphology and composition of the nucleus and nuclear envelope. It is also useful in studying changes in the nuclear structure during mitosis and meiosis. The antibody was raised using a nuclear pore complex mixture and was subsequently purified via Protein-G chromatography. It is believed to recognize the repeated FXFG repeat sequence in nuceloporins including p62, p152 and p90 and other proteins. |
| Uses | | This antibody is effective in immunoblotting, immunohistochemistry (IHC), immunofluorescence (IF), immunoprecipitation (IP) and immunoelectron microscopy (IEM). This antibody has been used successfully in frozen sections. |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition.
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| Notes | | *Predicted MW = 62kD |
| Storage | | Nuclear Pore Complex Proteins (MAb414) Monoclonal Antibody, Purified Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | | Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, Toshimori K, Nakano T. Mouse germ cell-less as an essential component for nuclear integrity Mol Cell Biol 23:1304-1315, 2003. (IHC) Lopez-Soler RI, Moir RD, Spann TP, Stick R, Goldman RD. A role for nuclear lamins in nuclear envelope assembly. J Cell Biol 154(1):61-70, 2001. Aris JP, Blobel G. Yeast nuclear envelope proteins cross-react with an antibody against mammalian pore complex proteins. J Cell Biol 108:2059-2067, 1989. Davis LI, Blobel G. Nuclear pore complex contains a family of glycoproteins that includes p62: Glycosylation through a previously unidentified cellular pathway.PNAS USA 84:7552-7556, 1987. Davis LI, Blobel G. Identification and characterization of a nuclear pore complex protein. Cell 45:699-709, 1986. Blobel G. Gene gating: a hypothesis. PNAS USA 82:8527-8529, 1985. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
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