| Description |  | Monoclonal Antibody against Detyrosinated α-Tubulin |
| Clone | | AA12 |
| Form | | Ascites |
| Host | | Mouse |
| Species Reactivity | | Mammalian |
| IsoType | | Detyrosinated α-Tubulin Monoclonal Antibody IgG2a |
| Specificity | | Microtubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of α- and β-tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. This antibody recognizes detyrosinated α-tubulin, a post translational modification of α-tubulin. This antibody was raised against the peptide sequence CGEEEGE. |
| Uses | | This antibody is effective in immunoblotting and immunofluorescence. |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. Covance antibodies may not be resold or modified for resale without prior written approval.
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| Notes | | *Predicted MW = 55 kD |
| Storage | | Detyrosinated α-Tubulin Monoclonal Antibody Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. |
| References | | Yoshiyama Y, Zhang B, Bruce J, Trojanowski JQ, Lee VM. Reduction of Detyrosinated Microtubules and Golgi Fragmentation Are Linked to Tau-Induced Degeneration in Astrocytes. J. Neurosci. 23:10662 - 10671, 2003. Mialhe A, Lafanechere L, Treilleux I, Peloux N, Dumontet C, Bremond A, Panh MH, Payan R, Wehland J, Margolis RL, Job D. Tubulin Detyrosination Is a Frequent Occurrence in Breast Cancers of Poor Prognosis. Cancer Res. 61:5024 - 5027, 2001. Idriss HT. Man to trypanosome: the tubulin tyrosination/detyrosination cycle revisited. Cell Motil Cytoskeleton 45(3):173-84, 2000. |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
