Stemgent® Affinity Purified anti-Human TRA-1-81 Antibody详细信息：
Product Overview
The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cellspecific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Stemgent® Affinity Purified anti-Human TRA-1-81 AntibodyProduct Specifications
Imunogen Human embryonal carcinoma cell line 2102Ep
Reactivity Human
Clone TRA-1-81
Isotype Mouse IgM, κ
Formulation Phosphate-buffered solution, pH 7.2, containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Storage Store undiluted at 4°C. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by flow cytometry and immunocytochemistry to ensure product quality.
Recommended Usage 0.25 μg per 1x106 viable cells for flow cytometry and 5 μg/ml for immunocytochemistry.
Stemgent® Affinity Purified anti-Human TRA-1-81 AntibodyTechnical Documents
Specifications
09-0011 Specifications Sheet
MSDS
Material Safety Data Sheet
Protocols
General Protocol for Immunocytochemistry
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