Stemgent® Affinity Purified anti-Mouse/Human Rex1 Antibody详细信息：
Product Overview
Affinity Purified anti-Mouse/Human Rex1 was screened on human and mouse ES cells using ICC and flow cytometry and selected as the best Rex1 antibody available for researchers needing to demonstrate pluripotency. Rex 1, also termed zinc-finger protein-42 (Zfp42), is a member of the YY1 sub-family of transcription factors1, and has emerged as an important stem cell marker in both mouse and human undifferentiated embryonic stem (ES) cells2. Expression of Rex1 is regulated by Nanog, Oct4, and Sox2 at the level of transcription1-3.
Stemgent® Affinity Purified anti-Mouse/Human Rex1 AntibodyReferences
Melissa B Rogers, Betsy A. Hosler, Lorraine J. Gudas. (1999) Specific Expression of a Retinoic Acid-Regulated, Zinc-Finger Gene, Rex-1, in Preimplantation Embryos, Trophoblast and Spermatocytes. Development 113:815-824.
Brivanlou AH, Gage FH, Jaenisch R, Jessell T, Melton D, Rossant J. (2003) Setting Standards for Human Embryonic Stem Cells. Science 300:913–916.
Wenjing Shi, Hui Wang, Guangjin Pan, Yijie Geng, Yunqian Guo, Duanqing Pei. (2006) Regulation of the Pluripotency Marker Rex-1 by Nanog and Sox2. The Journal of Biological Chemistry 281(33):23319–23325.
Product Specifications
Immunogen Synthetic mouse Rex1 peptide (within residues 1-100) conjugated to KLH
Reactivity Mouse and Human
Stemgent® Affinity Purified anti-Mouse/Human Rex1 AntibodyClone Polyclonal
Isotype Rabbit IgG
Formulation Phosphate-buffered solution pH 7.2 containing <0.1% sodium azide and <1.0% BSA.
Preparation The antibody was purified by immunogen affinity chromatography.
Storage Store undiluted at 4oC. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry (Figure 1) and flow cytometry (Figure 2).
Recommended Usage Dilute 1:100 for immunocytochemistry applications and 1:5 for flow cytometry applications.
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