Stemgent® Purified Mouse anti-Human CD34 Antibody详细信息：
Product Overview
Stemgent® Purified Mouse anti-Human CD34 Antibody was screened on normal human mesenchymal stem cells (MSCs) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD34 negative selection antibody for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification1-3. CD34 is a surface glycophosphoprotein expressed on developmentally early lymphohematopoietic stem and progenitor cells with a molecular weight of about 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands4-5.
Stemgent® Purified Mouse anti-Human CD34 AntibodyProduct Specifications
Immunogen Human peripheral leukocytes
Reactivity Human (not yet tested in MSC from other species)
Clone 4H11
Isotype Mouse IgG1, κ
Size 50 μg
Formulation Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Storage Store undiluted at 4°C. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Dilute 1:100 for immunocytochemistry and 1:20 for flow cytometry applications. For general protocols for performing ICC and FC directly on MSCs, see Technical Documents on the Stemgent®Purified Mouse anti-Human CD34 Antibody Product Page at www.stemgent.com/cat#09-0026.
Notice to Purchaser Purchaser represents and warrants that it will use the Stemgent®Purified Mouse anti-Human CD34 Antibody purely for research purposes, not for diagnostic use or resale. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
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