Stemgent® Purified Mouse anti-Human CD45 Antibody详细信息：
Product Overview
Stemgent® Purified Mouse anti-Human CD45 Antibody was screened on normal human mesenchymal stem cells (MSCs) by immunocytochemistry (ICC) and flow cytometry (FC) and selected as the best CD45 negative selection antibody for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification1-3. CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation4-5.
Stemgent® Purified Mouse anti-Human CD45 AntibodyProduct Specifications
Workshop Number IV N816
Reactivity Human (not yet tested in MSC from other species)
Clone HI30
Isotype Mouse IgG1, κ
Size 50 μg
Formulation Phosphate-buffered solution pH 7.2 containing ≤0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography.
Stemgent® Purified Mouse anti-Human CD45 AntibodyStorage Store undiluted at 4°C. Do not freeze.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested by immunocytochemistry and flow cytometry.
Recommended Usage Dilute 1:100 for immunocytochemistry and 1:20 for flow cytometry applications. For general protocols for performing ICC and FC directly on on MSC, see Technical Documents on the Stemgent®Purified Mouse anti-Human CD45 Antibody Product Page at www.stemgent.com/cat#09-0028.
Notice to Purchaser Purchaser represents and warrants that it will use the Stemgent® Purified Mouse anti-Human CD45 Antibody purely for research purposes, not for diagnostic use or resale. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
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