Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody详细信息：
Product Overview
Stemgent ® StainAlive™ Dylight 488™ Mouse anti-Human TRA-1-81 Antibody was screened on live, unfixed mouse embryonic stem (ES) cells using immunocytochemistry (ICC). This antibody was selected as the best conjugated TRA-1-81 antibody available for visualization of EC/ES/iPS cells, in fluorescent staining and real-time imaging without fixation or killing the cells. This antibody format is useful for direct visualization of the reprogramming process to iPS colonies. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide and is confirmed to be free of bacteria, fungi , mouse virus and mycoplasma as tested. The live ES cell morphology, proliferation and expression of pluripotency markers did not change after staining with this antibody. The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. As human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer.
Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 AntibodyProduct Specifications
Size / Quantity 50 µg (100 µl at 0.5 mg/ml)
Immunogen Human embryonal carcinoma cell line 2102Ep
Reactivity Human
Clone TRA-1-81
Isotype Mouse IgM,┃
Formulation Phosphate-buffered solution, pH 7.2, containing no sodium azide and 0.2 μm filter sterilized. Endotoxin level is < 1 EU/μg of the protein (< 0.1 ng/μg ) as determined by the LAL assay. It is free from bacteria, fungi, mouse virus and mycoplasma as tested.
Preparation The antibody was purified by affinity chromatography, and conjugated with Dylight™ 488 under optimal conditions. The solution is free of unconjugated dye and free antibody.
Storage and Handling Store at 4°C. Do not freeze. Light sensitive. This formulation contains no preservative. Handle under aseptic conditions.
Stability Stable for 6 months from date of receipt when stored as directed.
Quality Control Tested in immunocytochemistry (Figure 1) to ensure product quality.
Recommended Usage Use antibody at 1:100 in ICC. For general protocols for performing viable cell immunocytochemistry directly on stem cells, see the Technical Documents on the Stemgent® StainAlive™ Dylight™ 488 anti-Human TRA-1-81 product page at www.stemgent.com/cat#09-0069.
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Notice to Purchaser Purchaser represents and warrants that it will use Stemgent® StainAlive™ DyLight™ 488 Mouse anti-Human TRA-1-81 Antibody purely for research purposes, not for diagnostic use, not for resale, and not for use in humans or veterinary applications. Stemgent will not be held responsible for patent infringement or other violations that may occur with the use of our products.
Trademark DyLight™ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
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