| Description |  | Monoclonal Antibody against a Calcium Dependent Activation Epitope on Integrin β1 |
| Intended Use | | **Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | | P4G11 |
| Form | | Ascites |
| Host | | Mouse |
| Species Reactivity | | Integrin β1 (Activating) Monoclonal Antibody Human |
| IsoType | | IgG1 |
| [Ab] | | 2-3 mg/mL |
| Specificity | | The P4G11 monoclonal antibody was generated against IL-1β activated human umbilical vein endothelial cells (HUVECs). P4G11, reacts with calcium-dependent epitopes on the integrin β1 subunit. When bound to β1, P4G11, activates β1 dependent binding to extra cellular matrix substrates. |
| Uses | | This antibody is effective in immunoblotting, immunofluorescence (IF) and immunoprecipitation (IP). |
| Suggested Working Dilution | | The optimal working dilution should be determined for each specific assay condition.
|
| Storage | | Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | | Wayner EA, Hoffstrom BG. Monoclonal antibodies to integrin receptors. Meths. Enzymol. In: Integrins, edited by David Cheresh, 426:117-154, 2007. [General Use] Wayner EA, Gil SG, Murphy GF, Wilke MS, Carter WG. Epiligrin, a component of epithelial basement membranes, is an adhesive ligand for alpha 3 beta 1 positive T lymphocytes. J Cell Biol 121(5):1141-1152, 1993. [IP] |
| Warranty/Conditions | | Covance products may not be resold or modified for resale without prior written approval. |
