Cat.#P-6191
猪卵巢平滑肌细胞，Porcine Primary Ovarian Smooth Muscle Cells。每管细胞数量至少1x106cells/ml。
推荐培养基：M2268
运输条件： 干冰运输、 液氮保存
产品说明链接：http://www.cellbiologics.net/document/1421790917.pdf
Product DescriptionPorcine Primary Ovarian Smooth Muscle Cells from
Cell Biologics are isolated from ovarian tissue of Porcine. Porcine Primary Ovarian Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in
Cell Biologics Culture Complete Growth Medium generally for 3-7 days. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10
6 cells per ml and is delivered frozen. The cells are characterized by immunofluorescent method with antibodies to α-smooth muscle actin. Porcine Primary Ovarian Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by
Cell Biologics (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by
Cell Biologics)
. Repeated freezing and thawing of cells is not recommended.
Laboratory ApplicationsPorcine Primary Ovarian Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics ProductsCell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics ProductsPorcine Primary Ovarian Smooth Muscle Cells from
Cell Biologics are distributed fo
