CD-1 小鼠间充质干细胞，CD-1 Mouse Bone Marrow Mesenchymal Stem Cells

Cat.#CD-1043 
CD-1 小鼠间充质干细胞，CD-1 Mouse Bone Marrow Mesenchymal Stem Cells。每管细胞数量至少2x10⁶cells/ml。 

推荐培养基：M5566

运输条件： 干冰运输、 液氮保存

产品说明链接：http://cellbiologics.com/document/1426108993.pdf

Product Description
CD1 Mouse Bone Marrow Mesenchymal Stem Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice.  They are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium for 7-15 days. Cultures are then expanded.  Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials.  Each vial contains at least 2x10⁶ cells per ml and is delivered frozen. MBMMSCs are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
MBMMSCs are tested for expression of markers using antibodies, CD44, Sca-1 and CD29 by flow cytometry.
Laboratory Applications
MBMMSCs can be used in standard biochemical procedures include PCR, Western blotting, immunoprecipitation, or cell derivatives for desired research applications.
Storage of Cell Biologics Products
Cell Biologics ships frozen cells on dry ice.  On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.  Live-cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics Products
MBMMSCs from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures.  Transfer or resale of any Cell Biologics’ cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent.  Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Although MBMMSCs are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells withCell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.