Product Description
BALB/c Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are isolated from kidney glomerular tissue of pathogen-free laboratory mice. BALB/c Mouse Primary Kidney Glomerular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Cell Biologics’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106cells per ml and are delivered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody.
Product Testing
BALB/c Mouse Primary Kidney Glomerular Endothelial Cells are tested for expression of markers using antibody, VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz); AF1002 (R&D System) or CD31/PECAM-1 (Purified Rat Anti-Mouse CD31, Catalog No. 553370, BD) by immunofluorescence staining or FACS. BALB/c Mouse Primary Kidney Glomerular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified byCell Biologics. Repeated freezing and thawing of cells is not recommended.
Laboratory Applications
BALB/c Mouse Primary Kidney Glomerular Endothelial Cells can be used in assays of cell-cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Storage of Cell Biologics’ Products
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Authorized Uses of Cell Biologics’ Products
BALB/c Mouse Primary Kidney Glomerular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics’cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the company’s written consent.
Cell Biologics’ Terms and Conditions must be accepted before submitting an order.
Disclaimer
Although BALB/c Mouse Primary Kidney Glomerular Endothelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Warranty and Liability
Cell Biologics’ guarantee applies only to your purchase of Cell Biologics’ cells withCell Biologics’ Media and Coating Solution for appropriate cell culture and cell testing following Cell Biologics’ online protocols within 35 days from the date of product delivery.
