Stemolecule ROCK II Inhibitor is a specific inhibitor for type 2 Rho-associated, coiled-coil containing protein kinase (ROCK)1. ROCKs are serine/threonine-specific protein kinases which play a crucial role in organization of the actin cytoskeleton2. ROCK inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency2,3.
Product Specifications：2 mg
Alternate Name：N-(2-(2-(dimethylamino)ethoxy)-4-(1H-pyrazol-4-yl)phenyl)-2,3-dihydrobenzo[b][1,4]dioxine-2-carboxamide
Chemical Formula：C22H24N4O4
Molecular Weight：408.45
CAS Number：N/A
Purity：Greater than 98% by HPLC analysis
Formulation：White powder
Solubility：For a 10 mM concentrated stock solution of ROCK II Inhibitor, reconstitute the compound by adding 489.7 µl of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM.
Storage and Stability：Store powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.
Quality Control：The purity of ROCK II Inhibitor was determined by HPLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of ROCK II Inhibitor was tested on mouse embryonic stem cells.
