脑组织裂解物,Cerebrum Lysate

脑组织裂解物,Cerebrum Lysate(产品名称请以英文名称为准)【英文名称】:Cerebrum Lysate【货号】:1413【规格】:0.1 mg【保存条件】:Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.【简介】:Cerebrum tissue lysate was prepared by homogenization in modified RIPAbuffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mMethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1%Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris wasremoved by centrifugation. Protein concentration was determined withBio-Rad protein assay. The product was boiled for 5 min in 1 x SDSsample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodiumdodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.脑组织裂解物,Cerebrum Lysate 本公司多年专业提供科研试剂，质量保证，价格低，国家ZD实验室及国内各大院校长期指定供应商，工作时间内免费的技术咨询和指导，期待您的来电！