Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体抗原
Alstrom Syndrome 1 (ALMS1)
别名 ALMS1, ALSS, bbb
抗原表位
其他选择 C-Term
适用
其他选择 人
宿主
其他选择 山羊
克隆类型
多克隆
标记
其他选择 非结合性
实验类型 Peptide ELISA
Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体应用范围
其他选择 酶联免疫吸附测定（ELISA）, Western Blotting (WB), 免疫组织化学（IHC）
Pubmed 找到1个引用
规格 100 μg
发货至 ZG (更改)
发货时间 24至29个工作日
产品编号 yb184952
产品细节
免疫原 Peptide with sequence C-RVTNQLLGRKVPWD, from the C Terminus of the protein sequence according to NP_055935.4.
特异性 The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
交叉反应 小鼠, 大鼠, 犬, 牛
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
目标详细情况
别名 ALMS1
Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体背景 Synonyms: ALMS1, Alstrom syndrome 1, ALSS, KIAA0328, DKFZp686A118, DKFZp686D1828
基因ID 7840, 236266, 297408
NCBI登录号 NP_055935
研究领域 Immunology
使用细节
应用备注 ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 200 kDa and 75 kDa in lysates of Human T cell line Jurkat after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461 kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration: 1-3 µg/mL.
限制 仅限研究用
贮存及处理
状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.
