6-His human calretinin (recombinant) produced in Escherichia Coli.

This recombinant protein has been purified by affinity chromatography on a NTA-resin 
 
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, 
radioimmunoassay and intracellular injection.  
 
For adsorption we suggest the following procedure: 
 
-  Dilute 1 µl of the antiserum 7696 (SWant) against calretinin in 5 ml of the usual buffer for 
immunohistochemistry (final dilution 1:5'000). 
- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.  
- Incubate for at least 6 hours in the cold. 
- Apply to tissue-sections and incubate for 3 days. 
- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex,  DAB). 
 
As a result, the immunostaining should be strongly reduced or even completely prevented.